Product Name:
Tyk2-pY1054+pY1055
Product Number:
ab-pk845
Target Full Name: Protein-tyrosine kinase 2 (Jak-related)
Target Alias: JTK1; Kinase Tyk2; Tyrosine kinase 2; CCDS12236.1; ENSG00000105397
Product Type Specific: Tyk2 protein kinase phosphosite-specific antibody
Antibody Code: PK845
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1054+Y1055
Protein UniProt: P29597
Protein SigNET: Tyk2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Tyk2 sequence peptide Cat. No.: PE-04AHX99
Antibody Immunogen Sequence: GHE(pY)(pY)RVR(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G1051 to R1058; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. Y1054 and Y1055 phosphorylation stimulate phosphotransferase activity. The Y1054 site is one of the major in vivo phosphorylation sites in TYK2 (≥60 mass spectrometry reports recorded in PhosphoSitePlus), whereas T1055 appears to be phosphorylated less (≥7 mass spectrometry reports recorded in PhosphoSitePlus). TYK2 is known to be phosphorylated at this site in vitro by Y1054 and Y1055 by Tyk2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in A431 cells.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse | Frog .
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with JAK3 and JAK2, which feature similar phosphosite sequences. No significant cross-reactive proteins detected in A431 cells and HeLa cells.
Related Product 1: Tyk2 (1051-1058) pY1054+pY1055 antibody blocking peptide (Cat. No.: PE-04AHX99)
Related Product 2: Tyk2 expression antibody (Cat. No.: AB-NK181-3)
Related Product 3: Tyk2 expression antibody (Cat. No.: AB-NK181-4)
Related Product 4: Tyk2 expression antibody (Cat. No.: AB-NK181-5)
Related Product 5: Tyk2 Y292 phosphorylation antibody (Cat. No.: AB-PK846)
Related Product 6: JAK1 Y1034+Y1035 phosphorylation antibody (Cat. No.: AB-PK884)
Related Product 7: JAK1 T1107 phosphorylation antibody (Cat. No.: AB-PK895)
Related Product 8: JAK2 Y570 phosphorylation antibody (Cat. No.: AB-PK668)
Related Product 9: JAK2 Y1007+Y1008 phosphorylation antibody (Cat. No.: AB-PK667)
Related Product 10: JAK3 Y980+Y981 phosphorylation antibody (Cat. No.: AB-PK669)
Scientific Background: Tyk2 (Tyrosine kinase 2) is a 134 kDa protein-tyrosine kinase of the TK group and JakA family. It functions an intracellular signaling enzyme crucial for innate and adaptive immunity. It associates with type I/II cytokine receptors to mediate signals for interleukins and interferons, and regulates cell growth and inflammation. It mediates signaling through type I interferon receptors (IFNAR1), IL-12, IL-23, and IL-22 receptors. Upon cytokine binding, Tyk2 phosphorylates receptor subunits and STAT proteins, driving immune response regulation. It features tandem kinase domains: a C-terminal catalytic tyrosine kinase domain (JH1) and an allosteric pseudo-kinase domain (JH2). Phosphorylation of Tyr-1054 and Tyr-1055 increases phosphotransferase activity. Tyk2 is heavily implicated in autoimmune and inflammatory diseases, including psoriasis, psoriatic arthritis, systemic lupus erythematosus (SLE), and inflammatory bowel disease.Missense mutations in this gene have been found in colon and ovarian tumours. This description may include information annotated by UniProt and/or Google AI.