Product Name:
Tyk2-pY1054+pY1055
Product Number:
ab-pk845
Target Full Name: Protein-tyrosine kinase 2 (Jak-related)
Target Alias: JTK1; Kinase Tyk2; Tyrosine kinase 2; CCDS12236.1; ENSG00000105397
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK845
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1054+Y1055
Protein UniProt: P29597
Protein SigNET: P29597
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Tyk2 sequence peptide Cat. No.: PE-04AHX99
Antibody Immunogen Sequence: GHE(pY)(pY)RVR(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G1051 to R1058; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues G1051 to R1058; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 / 1.0 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in A431 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with JAK3 and JAK2, which feature similar phosphosite sequences. No significant cross-reactive proteins detected in A431 cells and HeLa cells.
Related Product 1: Tyk2-pY1054+pY1055 blocking peptide
Related Product 2: Tyk2-1 pan-specific antibody (Cat. No.: AB-NK181-3)
Related Product 3: Tyk2-3 pan-specific antibody (Cat. No.: AB-NK181-5)
Scientific Background: Tyk2 is a protein-tyrosine kinase of the TK group and JakA family. Phosphorylation of Y1054 and Y1055 increases phosphotransferase activity. Tyk2 mediates intracellular signal transduction from Type I interferon-alpha/beta receptor (IFN) signalling and phosphorylates the interferon-alpha/beta receptor alpha chain. Missense mutations in this gene have been found in colon and ovarian tumours.
