Product Name:
TXK-pY420
Product Number:
ab-pk844
Target Full Name: Tyrosine-protein kinase TXK
Target Alias: BTKL; MGC22473; PSCTK5; PTK4; RLK; TKL; ENSG00000074966
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK844
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y420
Protein UniProt: P42681
Protein SigNET: P42681
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TXK sequence peptide Cat. No.: PE-04AQI99
Antibody Immunogen Sequence: DDE(pY)VSS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D417 to S423
Production Method: Corresponds to amino acid residues D417 to S423
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.25 / 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-65 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in MCF7 cells, except insulin increases a strong 75 kDa protein signal. Only very low cross-reactivity was observed in A431,T98G and T98G cells.
Related Product 1: TXK-pY420 blocking peptide
Scientific Background: TXK is one of several protein kinases that can phosphorylate KinSub1MTMTP. TXK (RLK) is a protein-tyrosine kinase that is a member of the TK group of protein kinases in the Tec family. This kinase is moderate to highly expressed in most tested human tissues except in the brain, heart and spinal cord where it is poorly expressed. TXK has been shown to participate in multiple intracellular signalling pathways and thus influences many cellular processes, including the regulation of the actin cytoskeleton. TXK functions to regulate the development, activity, and differentiation of T-cells and NKT-cells. It is an important component of signalling pathways downstream of lymphocyte antigen receptor. TXK is phosphorylated in response to T-cell receptor stimulation and can be activated by phosphorylation by Src family kinases. The TXK protein is activated by phosphorylation at Y420. . Excessive TXK protein expression is seen in patients with Behcet's disease. Over production of TXK leads to increased Th1 cell function that is involved in the pathogenesis of Behcet's disease.
