Product Name:
TXK-pY420
Product Number:
ab-pk844
Target Full Name: Tyrosine-protein kinase TXK
Target Alias: BTKL; MGC22473; PSCTK5; PTK4; RLK; TKL; ENSG00000074966
Product Type Specific: TXK protein kinase phosphosite-specific antibody
Antibody Code: PK844
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y420
Protein UniProt: P42681
Protein SigNET: TXK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TXK sequence peptide Cat. No.: PE-04AQI99
Antibody Immunogen Sequence: DDE(pY)VSS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D417 to S423. Y420 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major sites of in vivo phosphorylation of TXK based on ≥13 mass spectrometry reports recorded in PhosphoSitePlus. TXK is known to be phosphorylated at this site in vitro by Fyn, and TXK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-65 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in MCF7 cells, except insulin increases a strong 75 kDa protein signal. Only very low cross-reactivity was observed in A431,T98G and T98G cells.
Related Product 1: TXK (417-423) pY420 antibody blocking peptide (Cat. No.: PE-04AQI99)
Scientific Background: TXK is one of several protein kinases that can phosphorylate KinSub1MTMTP. TXK (RLK) is a protein-tyrosine kinase that is a member of the TK group of protein kinases in the Tec family. This kinase is moderate to highly expressed in most tested human tissues except in the brain, heart and spinal cord where it is poorly expressed. TXK has been shown to participate in multiple intracellular signalling pathways and thus influences many cellular processes, including the regulation of the actin cytoskeleton. TXK functions to regulate the development, activity, and differentiation of T-cells and NKT-cells. It is an important component of signalling pathways downstream of lymphocyte antigen receptor. TXK is phosphorylated in response to T-cell receptor stimulation and can be activated by phosphorylation by Src family kinases. The TXK protein is activated by phosphorylation at Y420. . Excessive TXK protein expression is seen in patients with Behcet's disease. Over production of TXK leads to increased Th1 cell function that is involved in the pathogenesis of Behcet's disease. This description may include information annotated by UniProt and/or Google AI.