Product Name:
TTK-pT677
Product Number:
ab-pk842
Target Full Name: Dual specificity protein kinase TTK
Target Alias: Cancer/testis antigen 96; CT96; ESK; Esk1; FLJ38280; Mps1; MPS1L1; Phosphotyrosine picked threonine-protein kinase; PYT; TTK protein kinase; ENSG00000112742
Product Type Specific: TTK protein kinase phosphosite-specific antibody
Antibody Code: PK842
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S677
Protein UniProt: P33981
Protein SigNET: TTK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TTK sequence peptide Cat. No.: PE-04AQF99
Antibody Immunogen Sequence: DTT(pS)VVK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D674 to K680. T676 and likely S677 phosphorylation stimulates phosphotransferase activity. Phosphorylation regulates cell cycle progression and molecular association. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the less major sites of in vivo phosphorylation of TTK (≥1 mass spectrometry report in PhosphoSitePlus). TTK is known to be phosphorylated at this site in vitro by TTK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-103 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in T98G cells.
Related Product 1: TTK (674-680) pT676 antibody blocking peptide (Cat. No.: PE-04AQF99)
Related Product 2: TTKSubtide kinase substrate peptide (Cat. No.: PE-01BJR90)
Related Product 3: TTK-pY833+pY836 phosphosite-specific antibody (Cat. No.: AB-PK843)
Scientific Background: TTK (ESK) is a protein-serine/threonine kinase of the Other group and TTK family. It is a dual specificity protein kinase that can phosphorylate serine, threonine, and tyrosine residues and has a role in cell cycle progression through regulating centrosome duplication during S-phase. This function was CDK2-mediated by direct activation of TTK.. Through CDCA8 phosphorylation TTK can modulate AurKB at the centromere to align the chromosomes. Phosphorylation of S582, T675, T676, T686, and S742 increases phosphotransferase activity. Phosphorylation of T806 inhibits phosphotransferase activity. TTK is a dual-specificity protein kinase with activity toward serine, threonine and tyrosine residues. TTK was found to have a role Overexpression or dysregulation of TTK activtiy may promote cancer through aberrant duplication of centrosomes and promotiong of aneuploidy. Mutations in TTK are highly correlated in the development of microsatellite-unstable colorectal cancers. TTK is linked to pancreatic ductal adenocarcinoma (PDAC), and important in the growth and proliferation of PDAC cancer cells. Triple Negative Breast Cancer (TNBC) is a highly aggressive form of cancer and TTK is consistently upregulated in TNBC tissues. This description may include information annotated by UniProt and/or Google AI.