Product Name:
TSSK3-pT168
Product Number:
ab-pk841
Target Full Name: Testis-specific serine/threonine-protein kinase 3
Target Alias: SPOGA3; ST2C; STK22C; STK22C
Product Type Specific: TSSK3 protein kinase phosphosite-specific antibody
Antibody Code: PK841
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T168
Protein UniProt: Q96PN8
Protein SigNET: TSSK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TSSK3 sequence peptide Cat. No.: PE-04AQG99
Antibody Immunogen Sequence: LSQ(pT)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L165 to G171; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T168 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This is one of the only sites of in vivo phosphorylation of TSSK3 based on ≥1 mass spectrometry report in PhosphoSitePlus. TSSK3 is known to be phosphorylated at this site in vitro by PDPK1 (PDK1), and TSSK3 (STK22C).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 47-55 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HEK-293 and T98G cells, except for one cross-reactive protein of ~75 kDa.
Related Product 1: TSSK3 (165-171) pT168 antibody blocking peptide (Cat. No.: PE-04AQG99)
Scientific Background: TSSK3 (STK22C) is a protein-serine/threonine kinase of the CAMK group and TSSK family. It is activated by phosphorylation on T168, potentially by autophosphorylation. This description may include information annotated by UniProt and/or Google AI.