Product Name:
TrkB-pY516
Product Number:
ab-pk838
Target Full Name: BNDF/NT3/4/5 receptor- tyrosine kinase
Target Alias: GP145-TrkB; GP145-TrkB,GP95-TrkB; NTrk2; CCDS6671.1; ENSG00000148053
Product Type Specific: TrkB protein kinase phosphosite-specific antibody
Antibody Code: PK838
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y516
Protein UniProt: Q16620
Protein SigNET: TrkB
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TrkB (NTrk2) sequence peptide Cat. No.: PE-04AQD80
Antibody Immunogen Sequence: NPQ(pY)FGI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N513 to I519; in the region between the transmembrane domain and the protein kinase catalytic domain. Y516 phosphorylation stimulates phosphotransferase activity. This is a major site of phosphorylation for TrkB based on ≥42 mass spectrometry reports recorded in PhosphoSitePlus. TrkB is known to be phosphorylated at this site in vitro by TrkB (NTRK2).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-95 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Cross-reacts with prominent 37, 75-80, 130 and 160 kDa protein in sea star oocytes.
Related Product 1: TrkSubtide kinase substrate peptide (Cat. No.: PE-01BJQ95)
Related Product 2: TRKB (513-519) pY516 antibody blocking peptide (Cat. No.: PE-04AQD80)
Related Product 3: TrkA Y680+Y681 phosphorylation antibody (Cat. No.: AB-PK837)
Related Product 4: TrkB Y702 phosphorylation antibody (Cat. No.: AB-PK839)
Related Product 5: TrkB Y706+Y707 phosphorylation antibody (Cat. No.: AB-PK917)
Related Product 6: TrkC Y709+Y710 phosphorylation antibody (Cat. No.: AB-PK840)
Scientific Background: TrkB is one of several protein kinases that can phosphorylate KinSub5DDDYV. TrkB is a receptor protein-tyrosine kinase that is a member of the TK group of protein kinases in the Trk family. This kinase shows high variability in human tissue distribution with the highest levels detected in brain, ovary, pancreas, pituitary, spinal cord, thyroid and trachea. TrkB is activated by binding BDNF, NT-3, NT-4 or NT-5, which induce dimerization and autophosphorylation. Phosphorylation at Y516 increases phosphotransferase activity and induces interaction with PLCg1. TrkB activation stimulates signalling throught the MAPK pathway. Signalling through TrkB leads to cell differentiation. Mutations in the TrkB gene have been associated with obesity and mood disorders. TrkB has also been linked with the development of colon and lung cancers, neuroblastomas and pilocytic astrocytomas, which are benign tumours of the brain or spinal cord that grow slowly. This description may include information annotated by UniProt and/or Google AI.