Product Name:
TrkA-pY680+pY681
Product Number:
ab-pk837
Target Full Name: Nerve growth factor (NGF) receptor-tyrosine kinase; High affinity nerve growth factor receptor
Target Alias: DKFZp781I14186; MTC; NTrk1; P140-TrkA; Slow nerve growth factor receptor; Trk; Trk1; Trk4;Trk-A; p140-TrkA; Q04227; CCDS1161.1; Q04227; ENSG00000198400
Product Type Specific: TrkA protein kinase phosphosite-specific antibody
Antibody Code: PK837
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y680+Y681
Protein UniProt: P04629
Protein SigNET: TrkA
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TrkA (NGFR; NTrk1) sequence peptide Cat. No.: PE-04AHV95
Antibody Immunogen Sequence: STD(pY)(pY)RVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S677 to G684; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. Y680 and Y681 phosphorylation stimulate phosphotransferase activity. This are minor sites of phosphorylation for TrkA based on ≥10 and ≥6 high throughput mass spectrometry reports, respectively, recorded in PhosphoSitePlus. TrkA is known to be phosphorylated at this site in vitro by Y680 and Y681 by TrkA (NTRK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Tiger blowfish | Fruit fly.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.
Related Product 1: TrkSubtide kinase substrate peptide (Cat. No.: PE-01BJQ95)
Related Product 2: TRKA (677-684) pY680+pY681 antibody blocking peptide (Cat. No.: PE-04AHV95)
Related Product 3: TrkB Y516 phosphorylation antibody (Cat. No.: AB-PK838)
Related Product 4: TrkB Y702 phosphorylation antibody (Cat. No.: AB-PK839)
Related Product 5: TrkB Y706+Y707 phosphorylation antibody (Cat. No.: AB-PK917)
Related Product 6: TrkC Y709+Y710 phosphorylation antibody (Cat. No.: AB-PK840)
Scientific Background: TrkA (NTrk1) is a protein-tyrosine kinase of the TK group and Trk family. It is a receptor protein-tyrosine kinase with high affinity for nerve growth factor (NGF). The TrkA protein functions in the development and maturation of both the central nervous system (CNS) and peripheral nervous system (PNS) through the regulation of neuronal proliferation, differentiation, and survival, specifically of sympathetic neurons. Upon binding to NGF, TrkA undergoes homodimerization and activating autophosphorylation. Autophosphorylation of Y496 increases phosphotransferase activity and induces interaction with FRS2 and Shc1. Autophosphorylation of Y757 increases phosphotransferase activity. Autophosphorylation of Y791 increases phosphotransferase activity and induces interaction with PLCg1. Phosphorylation of Y701 inhibits phosphotransferase activity and induces interaction with CLTC and LAMP1. These interactions mediate NGF effects on cell survival and proliferation. TrkA appears to be an oncoprotein (OP). Overactive forms of the TrkA protein are known to have oncogenic properties and promote tumorigenesis. Chromosomal translocations involving the TrkA gene have been observed in thyroid papillary carcinoma tissue samples. This description may include information annotated by UniProt and/or Google AI.