Product Name:
TRIM28-pY458
Product Number:
ab-pk834
Target Full Name: Transcription intermediary factor 1-beta
Target Alias: KAP-1; KRIP-1; RING finger protein 96; RNF96; TIF1-beta; Tripartite motif-containing protein 28; TRIM28
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK834
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y458
Protein UniProt: Q13263
Protein SigNET: Q13263
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TRIM28 (TIF1B) sequence peptide Cat. No.: PE-04APZ99
Antibody Immunogen Sequence: DDP(pY)SSA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D455 to A461; In the region between the zf-B_box and PHD domains. One of the major in vivo sites of phosphorylation in TRIM28.
Production Method: Corresponds to amino acid residues D455 to A461; In the region between the zf-B_box and PHD domains. One of the major in vivo sites of phosphorylation in TRIM28.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.25 / 1.0 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-120 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins in A431, HeLa and MCF7 cells, except for a strong immunoreactive protein in MCF7 and A431 cells.
Related Product 1: TRIM28-pY458 blocking peptide
Related Product 2: TRIM28-pY517 phosphosite-specific antibody (Cat. No.: AB-PK835)
Scientific Background: TRIM28 (TIF1b)) is a protein-serine/threonine kinase of the Atypical group and TIF1 family. TIF family members are associated in retroviral restriction. TRIM28 contributes to EMT via E-cadherin and N-cadherin regulation in lung cancer cell lines.