Product Name:
TNK1-pY277
Product Number:
ab-pk832
Target Full Name: Non-receptor tyrosine-protein kinase TNK1
Target Alias: CD38 negative kinase 1; MGC46193; ENSG00000174292
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK832
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y277
Protein UniProt: Q13470
Protein SigNET: Q13470
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TNK1 sequence peptide Cat. No.: PE-04AQC80
Antibody Immunogen Sequence: RGR(pY)VMG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R274 to G280; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues R274 to G280; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 68-74 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins in HepG2 and MCF7 cells.
Related Product 1: TNK1-pY277 blocking peptide
Related Product 2: TNK1-CT pan-specific antibody (Cat. No.: AB-NK297-1)
Scientific Background: TNK1 is a protein-tyrosine kinase of the TK group and Ack family. It is a non-receptor kinase that functions as a negative regulator of cell growth through inhibition of the Ras-MAPK intracellular signalling pathway. TNK1 is thought to function in many tissues during fetal development and more selectively in the adult, with activity limited to cells of the lymphohematopoietic system. In addition, TNK1 is also predicted to play a role in phospholipid signalling. Phosphorylation of the TNK1 protein at several sites (Y77, Y235, Y277, Y287, and Y353) was observed in Hodgkin lymphoma (HL) cancer cell lines, indicating a possible role for the aberrant activity of the protein in the pathogenesis of this disease. In addition, a truncated form of TNK1 was identified in HL cancer cells. However, the effect of the truncation on its phosphotransferase activity was unclear.
