Product Name:
TNK1-pY277
Product Number:
ab-pk832
Target Full Name: Non-receptor tyrosine-protein kinase TNK1
Target Alias: CD38 negative kinase 1; MGC46193; ENSG00000174292
Product Type Specific: TNK1 protein kinase phosphosite-specific antibody
Antibody Code: PK832
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y277
Protein UniProt: Q13470
Protein SigNET: TNK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TNK1 sequence peptide Cat. No.: PE-04AQC80
Antibody Immunogen Sequence: RGR(pY)VMG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R274 to G280. Y277 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the minor sites of in vivo phosphorylation of TNK1 based on ≥4 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 68-74 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins in HepG2 and MCF7 cells.
Related Product 1: TNK1 (274-280) pY277 antibody blocking peptide (Cat. No.: PE-04AQC80)
Related Product 2: TNK1-CT pan-specific antibody (Cat. No.: AB-NK297-1)
Scientific Background: TNK1 is a protein-tyrosine kinase of the TK group and Ack family. It is a non-receptor kinase that functions as a negative regulator of cell growth through inhibition of the Ras-MAPK intracellular signalling pathway. TNK1 is thought to function in many tissues during fetal development and more selectively in the adult, with activity limited to cells of the lymphohematopoietic system. In addition, TNK1 is also predicted to play a role in phospholipid signalling. Phosphorylation of the TNK1 protein at several sites (Y77, Y235, Y277, Y287, and Y353) was observed in Hodgkin lymphoma (HL) cancer cell lines, indicating a possible role for the aberrant activity of the protein in the pathogenesis of this disease. In addition, a truncated form of TNK1 was identified in HL cancer cells. However, the effect of the truncation on its phosphotransferase activity was unclear. This description may include information annotated by UniProt and/or Google AI.