Product Name:
TIE2-pY992
Product Number:
ab-pk831
Target Full Name: Angiopoietin-1 receptor
Target Alias: Angiopoietin 1 receptor; CD202b antigen; HYK; P140 TEK; TEK; VMCM; VMCM1; OTTHUMP00000021167; TIE-2; hTIE2; ENSG00000120156
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK831
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y992
Protein UniProt: Q02763
Protein SigNET: Q02763
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TIE2 (TEK) sequence peptide Cat. No.: PE-04ALI90
Antibody Immunogen Sequence: QEV(pY)VKK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q989 to K995; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues Q989 to K995; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 135-150 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 cells.
Related Product 1: TIE2-pY992 blocking peptide
Related Product 2: TIE2-pY897 phosphosite-specific antibody (Cat. No.: AB-PK830)
Scientific Background: TIE2 (TEK) is a receptor protein-tyrosine kinase of the TK group and Tie family. It functions as a membrane receptor for ANGPT1, ANGPT2, and ANGPT4 and is involved in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion, actin cytoskeleton dynamics, and vascular quiescence. TIE2 expression is required for the normal development of the heart and blood vessels and for hematopoiesis post-natally. After birth, TIE2 expression can either promote or inhibit angiogenesis dependent upon the cellular context. In addition, TIE2 also has anti-inflammatory activity by preventing pro-inflammatory plasma proteins and leukocytes from leaking through the blood vessel endothelium. TIE2 directly phosphorylates DOK2, GRB7, GRB14, PIK3R1, Shc1, and TIE1 to mediate its effects. In mice, rats, and humans, TIE2 expression is almost exclusively in endothelial cells that line the vasculature. TIE2 has been demonstrated to be essential for the maintenance of hematopoeitic stem cells (HSCs) in a quiescent state in the bone marrow, which is required for the ongoing process of hematopoeisis. Phosphorylation at Y897 inhibits TIE2 phosphotransferase activity.
