Product Name:
TEC-pY519
Product Number:
ab-pk829
Target Full Name: Tyrosine-protein kinase TEC
Target Alias: Kinase TEC; MGC126760; MGC126762; PSCTK4; CCDS3481.1; P42680; ENSG00000135605
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK829
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y519
Protein UniProt: P42680
Protein SigNET: P42680
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TEC sequence peptide Cat. No.: PE-04ALH99
Antibody Immunogen Sequence: DDQ(pY)TSS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D516 to S522; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues D516 to S522; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in HepG2 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 65-75 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Some weak cross-reactivities in HepG2 cells, but no significant cross-reactivities in Jurkat cells and sea star oocytes.
Related Product 1: TEC-pY519 blocking peptide
Scientific Background: TEC is a non-receptorprotein-tyrosine kinase of the TK group and TEC family. It is an important component of T cell signalling and T cell activation in the adaptive immune response. When T-cells or B-cells receptors are activated, this leads to the recruitment of TEC to the cell membrane, where it is phosphorylated at Y519. It regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells, and osteoclast differentiation. It phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signalling. It mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. It plays a redundant role to BTK in BCR-signalling for B-cell development and activation, via phosphorylation of STAP1. It is involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. TEC phosphorylates GRB10. TEC is involved in G protein-coupled receptor- and integrin-mediated signallings in blood platelets. It plays a role in hepatocyte proliferation and liver regeneration and in HGF-induced ERK signalling. SOCS1 is an inhibitor of TEC kinase activity. TEC is a major binder of dasatinib, which is used for BCR/ABL-positive CML treatment.
