Product Name:
TBK1-pS172
Product Number:
ab-pk828
Target Full Name: TANK binding kinase TBK1; Serine/threonine-protein kinase TBK1
Target Alias: FLJ11330; Kinase TBK1; NAK; NF-KB-activating kinase NAK; TANK binding kinase TBK1; TANK-binding kinase 1; CCDS8968.1; ENSG00000183735
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK828
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S172
Protein UniProt: Q9UHD2
Protein SigNET: Q9UHD2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TBK1 sequence peptide Cat. No.: PE-04ALG90
Antibody Immunogen Sequence: QFV(pS)LYG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q169 to G175; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues Q169 to G175; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in HepG2 cellsStrong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-90 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected with HepG2 cells. Strongly cross-reacts with a ~55 kDa protein in sea star oocytes.
Related Product 1: TBK1-pS172 blocking peptide
Related Product 2: TBK1Subtide - TBK1 protein kinase substrate peptide
Scientific Background: TBK1 (NAK) is a protein-serine/threonine kinase of the Other group and IKK family. It plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. To establish such an antiviral state. It mediates the immune response via signalling with TANK, TRAF3, IRF3, IRF7, and DDX3X. It can act as a scaffold for the proteins FADD, AZI2, MAVS, TRADD, TANK or TBKBP1/SINTBAD. TBK1 can be ubiquitinated at K30 and K401. TBK1 is phosphorylated and activated at S172A, Y325, and Y577. It can phosphorylate NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. It phosphorylates and activates AKT1.
