Product Name:
TBK1-pS172
Product Number:
ab-pk828
Target Full Name: TANK binding kinase TBK1; Serine/threonine-protein kinase TBK1
Target Alias: FLJ11330; Kinase TBK1; NAK; NF-KB-activating kinase NAK; TANK binding kinase TBK1; TANK-binding kinase 1; CCDS8968.1; ENSG00000183735
Product Type Specific: TBK1 protein kinase phosphosite-specific antibody
Antibody Code: PK828
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S172
Protein UniProt: Q9UHD2
Protein SigNET: TBK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TBK1 sequence peptide Cat. No.: PE-04ALG90
Antibody Immunogen Sequence: QFV(pS)LYG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q169 to G175; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S172 phosphorylation stimulates phosphotransferase activity. This is the major site of in vivo phosphorylation of TBK1 based on ≥53 mass spectrometry reports recorded in PhosphoSitePlus. TBK1 is known to be phosphorylated at this site in vitro by TBK1 (NAK).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in HepG2 cellsStrong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Frog | Honey bee.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-90 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected with HepG2 cells. Strongly cross-reacts with a ~55 kDa protein in sea star oocytes.
Related Product 1: TBK1 (169-175) pS172 antibody blocking peptide (Cat. No.: PE-04ALG90)
Related Product 2: TBK1Subtide kinase substrate peptide (Cat. No.: PE-01BJO90)
Related Product 3: TBK1 S716 phosphorylation antibody (Cat. No.: AB-PK949)
Scientific Background: TBK1 (NAK) is a protein-serine/threonine kinase of the Other group and IKK family. It plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. To establish such an antiviral state. It mediates the immune response via signalling with TANK, TRAF3, IRF3, IRF7, and DDX3X. It can act as a scaffold for the proteins FADD, AZI2, MAVS, TRADD, TANK or TBKBP1/SINTBAD. TBK1 can be ubiquitinated at K30 and K401. TBK1 is phosphorylated and activated at S172A, Y325, and Y577. It can phosphorylate NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. It phosphorylates and activates AKT1. Mutations in TBK1, as identified in ALS, caused a loss of kinase function (1) and TBK1 knockout mice showed dendritic swellings, abnormally shaped astrocytes, and p62- and ubiquitin-positive aggregates in the cerebellum (2). Families with TBK1 mutations showed an increased risk to develop cognitive defects in addition to their motor symptoms (3), and ALS patients with TBK1 mutations displayed a bulbar onset more frequently (4). One ALS-associated protein that appear to be substrates for TBK1 is SQSTM1, and its phosphorylation facilitates its binding to polyubiquitinated proteins and efficient targeting of these proteins for degradation in autophagosomes (5). Another ALS-related protein that is a TBK1 target is OPTN, which is highly abundant, and is involved in the inflammatory response, autophagy, Golgi maintenance, and vesicular transport. Loss of function TBK1 mutations may affect ubiquitin-directed breakdown of aggregates through decreased OPTN targeting (6). This description may include information annotated by UniProt and/or Google AI.