Product Name:
TAOK1-pS181
Product Number:
ab-pk826
Target Full Name: Serine/threonine-protein kinase TAO1
Target Alias: FLJ14314; HKFC-B; KIAA1361; MAP3K16; MARKK; PSK2; STE20-like kinase PSK2; TAOK1; TAO kinase 1; KFC-B; MARKK; h KFC-B; ENSG00000160551
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK826
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S181
Protein UniProt: Q7L7X3
Protein SigNET: Q7L7X3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TAO1 (TAOK1) sequence peptide Cat. No.: PE-04ALF99
Antibody Immunogen Sequence: PAN(pS)FVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues P178 to G184; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues P178 to G184; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak to medium immunoreactivity of a target-sized protein by Western blotting in HeLa cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110-125 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities except for a~ 77 kDa protein in HEK-293, HeLa, Jurkat and T98G cells.
Related Product 1: TAO1-pS181 blocking peptide
Related Product 2: TAO1-pY309 phosphosite-specific antibody (Cat. No.: AB-PK827)
Related Product 3: TAOSubtide - TAOK2 (PSK) protein kinase substrate peptide
Scientific Background: TAO1 (MAP3K16, TAOK1) is a protein-serine/threonine kinase of the STE group and STE20 family. It is involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. It phosphorylates MAP2K3, MAP2K6 and MARK2, and acts as an activator of the p38 (MAPK14) stress-activated MAPK cascade by phosphorylation and activation of the upstream MKK3 and MKK6 kinases. It is involved in G protein-coupled receptor signalling to p38 (MAPK14). In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38 (MAPK14) stress-activated MAPK cascade, probably by mediating phosphorylation of MKK3 (MAP2K3) and MKK6 (MAP2K6). It serves as a regulator of cytoskeleton stability by phosphorylating MARK2 at T208, leading to its activation and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. It also regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the JNK (MAPK8) cascade.
