Product Name:
SRPK1-pS222
Product Number:
ab-pk819
Target Full Name: Serine/arginine-rich protein-specific kinase 1; SRPK1a protein kinase
Target Alias: SFRS protein kinase 1; SFRSK1; SRPK1a protein kinase
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK819
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S222
Protein UniProt: Q96SB4
Protein SigNET: Q96SB4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SRPK1 sequence peptide Cat. No.: PE-04APR99
Antibody Immunogen Sequence: ILG(pS)DDD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues I219 to D225; Near the N-terminus of the kinase.
Production Method: Corresponds to amino acid residues I219 to D225; Near the N-terminus of the kinase.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-100 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: In HeLa cells, phenylarsine oxide (PAO) increases detection of 80 and 75 kDa proteins. No cross-reactivities observed in T98G cells.
Related Product 1: SRPK1-pS222 blocking peptide
Related Product 2: SRPK2-IKCD pan-specific antibody (Cat. No.: AB-NK296-1)
Related Product 3: SRPKSubtide - SRPK1 protein kinase substrate peptide
Scientific Background: SRPK1 (SFRSK1) is one of several protein kinases that can phosphorylate KinSub5RRLSP. It is a protein-serine/threonine kinase of the CMGC group and SRPK family. This kinase is moderate to highly expressed in most tested human tissues except apparently in the brain and spinal cord. SRPK1 is activated by phosphorylation at S51 and S555. In human cancer cells, it was observed that inactivation of SRPK1 induces cellular resistance to anticancer drugs such as cisplatin and bleomycin. SRPK1 phosphorylates serine/arginine-rich (SR) proteins, such as splicing factors ASF/SF2, SC35, and SRp55, in their arginine/serine-rich (RS) domains in vitro. Consequently, it is believed that SRPK1 plays a key role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors.