Product Name:
SMG1-pT3550
Product Number:
ab-pk817
Target Full Name: Serine/threonine-protein kinase SMG1; Lambda/iota protein kinase C-interacting protein
Target Alias: ATX; KIAA0421; Lambda/iota protein kinase C-interacting protein; LIP
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK817
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T3550
Protein UniProt: Q96Q15
Protein SigNET: Q96Q15
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SMG1 sequence peptide Cat. No.: PE-04APQ99
Antibody Immunogen Sequence: GQK(pT)QPD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G3547 to D3553; In the C-terminus of SMG1. This is one of the two major in vivo sites for phosphorylation of SMG1.
Production Method: Corresponds to amino acid residues G3547 to D3553; In the C-terminus of SMG1. This is one of the two major in vivo sites for phosphorylation of SMG1.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 / 1.0 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 350-410 kDa.
Related Product 1: SMG1-pT3550 blocking peptide
Related Product 2: SMG1 pan-specific antibody (Cat. No.: AB-NK233)
Related Product 3: SMG1-1 pan-specific antibody (Cat. No.: AB-NK233-2)
Related Product 4: SMG1-2 pan-specific antibody (Cat. No.: AB-NK233-1)
Related Product 5: SMG1-3 pan-specific antibody (Cat. No.: AB-NK233-3)
Scientific Background: SMG1 (ATX) is a protein-serine/threonine kinase of the Atypical group and PIKK family. It mediates the genotoxic stress pathway as well as the mRNA surveillance pathway. SMG1 will target nonsense RNA molecules for degradation via phosphorylation of RENT1/UPF1. SMG1 is essential for optimal phosphorylation and activity of p53/TP53 following genotoxic stress. It is iInhibited by caffeine, LY294002 and wortmannin.
