Product Name:
SLK-pS189
Product Number:
ab-pk816
Target Full Name: STE20-like serine/threonine-protein kinase; CTCL tumour antigen se20-9
Target Alias: bA16H23.1; KIAA0204; LOSK; MGC133067; Se20-9; STE20-like kinase; STK2
Product Type Specific: SLK protein kinase phosphosite-specific antibody
Antibody Code: PK816
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S189
Protein UniProt: Q9H2G2
Protein SigNET: SLK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SLK sequence peptide Cat. No.: PE-04ALC99
Antibody Immunogen Sequence: RRD(pS)FIG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R186 to G192; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S189 phosphorylation is stimulatory for phosphotransferase activity. It is one of the major in vivo phosphorylation sites in SLK based on ≥344 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken .
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-200 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293, HeLa and Jurkat cells, except a 50 kDa protein in A431 cells and 250 kDa protein in HEK-293 and MCF7 cells.
Related Product 1: SLK (186-192) pS189 antibody blocking peptide (Cat. No.: PE-04ALC99)
Related Product 2: SMG1-1 pan-specific antibody (Cat. No.: AB-NK233-2)
Related Product 3: SMG1-2 pan-specific antibody (Cat. No.: AB-NK233-1)
Related Product 4: SMG1-3 pan-specific antibody (Cat. No.: AB-NK233-3)
Related Product 5: SMG1-pT3550 phosphosite-specific antibody (Cat. No.: AB-PK817)
Scientific Background: SLK (Ste20-like kinase) is a protein-serine/threonine kinase of the STE group and STE20 family. It is a ubiquitously expressed serine/threonine protein kinase involved in regulating essential cellular functions, including apoptosis, cell cycle progression, cytoskeletal organization, and cell migration. It regulates focal adhesion turnover and polarization of microtubules, which are critical for cell migration. It may function in apoptosis and actin stress fiber dissolution. it acts as a caspase-3-activated kinase that helps reorganize actin stress fibers and stabilize microtubules. It features an N-terminal catalytic kinase domain (approx. amino acids 1–338), a central regulatory region, and a C-terminal coiled-coil domain. It is found in all human tissues, with highest expression in skeletal muscle, heart, and kidney. SLK is crucial for development; its knockout results in embryonic lethality. It appears to be necessary for ErbB2-driven breast cancer cell motility. This description may include information annotated by UniProt and/or Google AI.