Product Name:
SLK-pS189
Product Number:
ab-pk816
Target Full Name: STE20-like serine/threonine-protein kinase; CTCL tumour antigen se20-9
Target Alias: bA16H23.1; KIAA0204; LOSK; MGC133067; Se20-9; STE20-like kinase; STK2
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK816
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S189
Protein UniProt: Q9H2G2
Protein SigNET: Q9H2G2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SLK sequence peptide Cat. No.: PE-04ALC99
Antibody Immunogen Sequence: RRD(pS)FIG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R186 to G192; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues R186 to G192; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-200 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293, HeLa and Jurkat cells, except a 50 kDa protein in A431 cells and 250 kDa protein in HEK-293 and MCF7 cells.
Related Product 1: SLK-pS189 blocking peptide
Related Product 2: SMG1-1 pan-specific antibody (Cat. No.: AB-NK233-2)
Related Product 3: SMG1-2 pan-specific antibody (Cat. No.: AB-NK233-1)
Related Product 4: SMG1-3 pan-specific antibody (Cat. No.: AB-NK233-3)
Related Product 5: SMG1-pT3550 phosphosite-specific antibody (Cat. No.: AB-PK817)
Scientific Background: SLK is a protein-serine/threonine kinase of the STE group and STE20 family. It may function in apoptosis and actin stress fiber dissolution. SLK was shown to be necessary for ErbB2-driven breast cancer cell motility.
