Product Name:
SIK3-pT411
Product Number:
ab-pk815
Target Full Name: Salt-inducible serine/threonine-protein kinase SIK3
Target Alias: FLJ12240; KIAA0999; L19; Salt-inducible kinase 3; Serine/threonine-protein kinase QSK; SIK family kinase 3; SIK3; SIK-3; L19; KIAA0999; ENSG00000160584
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK815
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T411
Protein UniProt: Q9Y2K2
Protein SigNET: Q9Y2K2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SIK3 (QSK) sequence peptide Cat. No.: PE-04APG99
Antibody Immunogen Sequence: RRH(pT)VGV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R408 to V414; In the first third of the protein. This is one of the two major in vivo sites for phosphorylation of SIK3.
Production Method: Corresponds to amino acid residues R408 to V414; In the first third of the protein. This is one of the two major in vivo sites for phosphorylation of SIK3.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110-115 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in T98G cells.
Related Product 1: SIK3-pT411 blocking peptide
Related Product 2: SIK3-1 pan-specific antibody (Cat. No.: AB-NK250-1)
Related Product 3: SIK3-2 pan-specific antibody (Cat. No.: AB-NK250-2)
Related Product 4: SIK3-3 pan-specific antibody (Cat. No.: AB-NK250-3)
Related Product 5: SIK3-pT163 phosphosite-specific antibody (Cat. No.: AB-PK814)
Scientific Background: SIK3 (QSK) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is a member of the salt-inducible kinase family. SIK3 is activated by phosphorylation on T163 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. It is a AMPK-family kinase that is ubiquitously expressed in the cytoplasm. In ovarian cancer, overexpression of SIK3 promotes G1/S cell cycle progression and cell proliferation and may therefore function as an oncoprotein.
