Product Name:
SIK3-pT163
Product Number:
ab-pk814
Target Full Name: Salt-inducible serine/threonine-protein kinase SIK3
Target Alias: FLJ12240; KIAA0999; L19; Salt-inducible kinase 3; Serine/threonine-protein kinase QSK; SIK family kinase 3; SIK3; SIK-3; L19; KIAA0999; ENSG00000160584
Product Type Specific: SIK3 (QSK) protein kinase phosphosite-specific antibody
Antibody Code: PK814
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T163
Protein UniProt: Q9Y2K2
Protein SigNET: SIK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SIK3 (QSK) sequence peptide Cat. No.: PE-04APF99
Antibody Immunogen Sequence: LLK(pT)WCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L160 to G166. T163 phosphorylation is predicted to stimulate phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is a major site of in vivo phosphorylation site of SIK3 based on ≥27 high throughput mass spectrometry reports recorded in PhosphoSitePlus. SIK3 is known to be phosphorylated at this site in vitro by LKB1 (STK11).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of target-sized protein in maturing sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Mouse | Fruit fly.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110-115 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in Jurkat cells.
Related Product 1: QSK (160-166) pT163 antibody blocking peptide (Cat. No.: PE-04APF99)
Related Product 2: SIK3-1 pan-specific antibody (Cat. No.: AB-NK250-1)
Related Product 3: SIK3-2 pan-specific antibody (Cat. No.: AB-NK250-2)
Related Product 4: SIK3-3 pan-specific antibody (Cat. No.: AB-NK250-3)
Related Product 5: SIK3-pT411 phosphosite-specific antibody (Cat. No.: AB-PK815)
Scientific Background: SIK3 (Salt-Inducible Kinase 3; QSK) is a 140 kDa protein-serine/threonine kinase of the CAMK group and CAMKL family. It is a member of the salt-inducible kinase family. It phosphorylates key substrates to manage energy storage, lipid metabolism, and cholesterol synthesis. It also positively regulates mTOR signaling by triggering the degradation of the inhibitor DEPTOR. SIK3 is crucial for homeostatic sleep/wake regulation, where a conserved phosphorylation site (Ser-551 in humans) regulates daily nonrapid-eye movement sleep amounts. It also controls the cell cycle by regulating HDAC4 trafficking. SIK3 is activated by phosphorylation on Thr-163 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. It is a AMPK-family kinase that is ubiquitously expressed in the cytoplasm. It is widely expressed in tissues like the liver and brain. SIK3 acts as a tumour-associated antigen and plays complex roles in cancer biology, including facilitating metastatic traits in breast cancer and acting downstream of LKB1 in lung cancer. In ovarian cancer, overexpression of SIK3 promotes G1/S cell cycle progression and cell proliferation. This description may include information annotated by UniProt and/or Google AI.