Product Name:
SIK2-pS358
Product Number:
ab-pk813
Target Full Name: Salt-inducible serine/threonine-protein kinase SIK2; Serine-threonine-protein kinase SNF1-like kinase 2
Target Alias: DKFZp434K1115; KIAA0781; LOH11CR1I; Salt-inducible kinase 2; Salt-inducible serine/threonine kinase 2; SIK2; SN1L2; SNF1LK2
Product Type Specific: SIK2 (QIK) protein kinase phosphosite-specific antibody
Antibody Code: PK813
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S358
Protein UniProt: Q9H0K1
Protein SigNET: SIK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SIK2 (QIK) sequence peptide Cat. No.: PE-04APE95
Antibody Immunogen Sequence: RRP(pS)TIA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R355 to A361; After the kinase catalytic domain. S358 phosphorylation stimulates phosphotransferase activity. This is one of the major sites of in vivo phosphorylation of SIK2 based on ≥143 mass spectrometry reports recorded in PhosphoSitePlus. SIK2 is known to be phosphorylated at this site in vitro by Akt1 (PKBa), Akt2 (PKBb), PKACa (PRKACA), and SIK2 (QIK).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-150 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa, MCF7 and T98G cells, but strong cross-reactivity with 75 kDa protein in sea star oocytes.
Related Product 1: SIK2-1 pan-specific antibody (Cat. No.: AB-NK249-1)
Related Product 2: SIK2-2 pan-specific antibody (Cat. No.: AB-NK249-2)
Related Product 3: SIK2-3 pan-specific antibody (Cat. No.: AB-NK249-3)
Scientific Background: SIK2 (Salt-inducible kinase 2; QIK) is a 94 kDa protein-serine/threonine kinase of the CAMK group and CAMKL family. It is a member of the salt-inducible kinase family. It regulates insulin signaling, adipocyte differentiation, and fat/cholesterol synthesis, with high expression in skeletal muscle and adipose tissue. SIK2 may modulate the efficiency of insulin signal transduction, and affect insulin resistancy. It regulates autophagy when proteasomal function is compromised and interacts with p97/VCP to control endoplasmic reticulum-associated protein degradation (ERAD). It features a kinase domain at the N-terminal, a central SNF1-protein kinase homology (SNH) domain, and a phosphorylation domain near the C-terminal. It is activated by phosphorylation on Thr-175 by LKB1 (STK11) in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. SIK2 kinase activity is regulated by an acetylation-deacetylation switch; p300/CBP-mediated acetylation at Lys-53 inhibits it, while HDAC6-mediated deacetylation restores it. SIK2 stimulates histone deacetylases export. SIK2 is a centrosome kinase required for bipolar mitotic spindle formation, and may be a potential target for therapy in ovarian cancer, where it promotes glycolysis and tumor growth via the PI3K/AKT signaling pathway. This description may include information annotated by UniProt and/or Google AI.