Product Name:
SgK269-pY635
Product Number:
ab-pk811
Target Full Name: Tyrosine-protein kinase SgK269; Pseudopodium-enriched atypical kinase 1
Target Alias: FLJ21140; FLJ34483; PEAK1; KIAA2002 protein (partial sequence); SG269; Tyrosine-protein kinase SgK269: Sugen kinase 269
Product Type Specific: SgK269 protein kinase phosphosite-specific antibody
Antibody Code: PK811
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y635
Protein UniProt: Q9H792
Protein SigNET: SgK269
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SgK269 (PEAK1) sequence peptide Cat. No.: PE-04AHC99
Antibody Immunogen Sequence: PNA(pY)DNL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues P632 to L638; in the N-terminal half of the protein. Y635 phosphorylation induces interaction with Grb2 and stimulates cells growth and motility. This is the major in vivo site for phosphorylation of Sgk269 based on ≥1218 mass spectrometry reports recorded in PhosphoSitePlus. SgK269 is known to be phosphorylated at this site in vitro by Lyn.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Platypus | Chicken | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 190-210 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in A431 cells.
Related Product 1: SgK269-pY635 blocking peptide
Scientific Background: SgK269 (PEAK1, pseudopodium-enriched atypical kinase 1) is a protein-serine/threonine kinase of the Other group and NKF3 family. It appears to function in the regulation of cell spreading and migration on fibronectin substrates. In addition, SgK269 is predicted to either directly or indirectly affect the phosphorylation of cytoskeleton-associated proteins, such as MAPK1/ERK and PXN. SgK269 has been demonstrated to function downstream of eIF5A1 and eIF5A2, translation initiation factors that have been implicated in several forms of human cancer. For example, the expression of these factors is required for the growth of pancreatic ductal adenocarcinoma (PDAC) cells in vivo and their overexpression in mice models leads to enhanced PDAC cell growth and tumour formation. Downstream activity of PEAK1 is required for the PDAC growth promoting effects of the eIF5A factors, indicating a role for SgK269 in the tumorigenesis of PDAC. This description may include information annotated by UniProt and/or Google AI.