Product Name:
SCYL1-pS754
Product Number:
ab-pk809
Target Full Name: N-terminal kinase-like protein
Target Alias: Coated vesicle-associated kinase of 90 kDa; SCY1-like protein 1; Telomerase regulation-associated protein; Telomerase transcriptional element-interacting factor; Teratoma-associated tyrosine kinase; GKLP; MGC78454; NKTL; NTKL; P105; TAPK; TEI; TRAP
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK809
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S754
Protein UniProt: Q96KG9
Protein SigNET: Q96KG9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SCYL1 sequence peptide Cat. No.: PE-04APN99
Antibody Immunogen Sequence: RPD(pS)WGE(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R751 to E757; In the C-terminus. This is the major in vivo phosphorylation site.
Production Method: Corresponds to amino acid residues R751 to E757; In the C-terminus. This is the major in vivo phosphorylation site.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-105 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2, except insulin decreases 48K; No significant cross-reactivities was detected in T98G cells. A medium-strong ~40 kDa protein was detected in HepG2 cells, and several weak cross-reactive proteins were observed in A431 cells.
Related Product 1: SCYL1-pS754 blocking peptide
Scientific Background: SCYL1 is a protein-serine/threonine kinase of the Other group and SCY1 family. It is apparently devoid of any measurable phosphotransferase activity in vitro, but its isoform (6) can bind 3 GC-rich sequences (box -A, -B, or -C) in the beta-polymerase promoter region.
