Product Name:
SCYL1-pS754
Product Number:
ab-pk809
Target Full Name: N-terminal kinase-like protein
Target Alias: Coated vesicle-associated kinase of 90 kDa; SCY1-like protein 1; Telomerase regulation-associated protein; Telomerase transcriptional element-interacting factor; Teratoma-associated tyrosine kinase; GKLP; MGC78454; NKTL; NTKL; P105; TAPK; TEI; TRAP
Product Type Specific: SCYL1 protein kinase phosphosite-specific antibody
Antibody Code: PK809
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S754
Protein UniProt: Q96KG9
Protein SigNET: SCYL1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SCYL1 sequence peptide Cat. No.: PE-04APN99
Antibody Immunogen Sequence: RPD(pS)WGE(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R751 to E757; in the C-terminus. S754 phosphorylation inhibits exocytosis. This is the major in vivo phosphorylation site of SCYL1 based on ≥90 mass spectrometry reports recorded in PhosphoSitePlus. SCYL1 is known to be phosphorylated at this site in vitro by mTor.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-105 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2, except insulin decreases 48K; No significant cross-reactivities was detected in T98G cells. A medium-strong ~40 kDa protein was detected in HepG2 cells, and several weak cross-reactive proteins were observed in A431 cells.
Related Product 1: SCYL1-pS754 blocking peptide
Scientific Background: SCYL1 (SCY1-like pseudokinase 1) is a protein-serine/threonine kinase of the Other group and SCY1 family. It is a multifunctional protein that acts as a transcriptional regulator and a key component in intracellular transport, particularly in COPI-mediated retrograde transport between the Golgi apparatus and endoplasmic reticulum. As a catalytically inactive pseudokinase, it regulates Golgi morphology, tRNA nuclear/cytoplasmic trafficking, and gene expression. Although it is apparently devoid of any measurable phosphotransferase activity in vitro, its isoform (6) can bind 3 GC-rich sequences (box -A, -B, or -C) in the beta-polymerase promoter region. SCYL1 features an N-terminal kinase-like domain (catalytically inactive), central HEAT repeats, and a C-terminal coiled-coil domain. It functions as a scaffold or regulator in COPI-mediated transport by binding directly to COPI coats, crucial for Golgi homeostasis. It acts as a transcriptional activator for telomerase reverse transcriptase (TERT) and DNA polymerase beta genes, often binding to GC-rich DNA sequences (e.g., via the TEIF domain). It is ocated in the cytoplasm, Golgi apparatus, and nucleus. Mutations in SCYL1 are linked to SCAR21 (spinocerebellar ataxia), a disorder featuring cerebellum degeneration, ataxia, and liver dysfunction. Overexpression is associated with cancer progression and poor prognosis in breast cancer. This description may include information annotated by UniProt and/or Google AI.