Product Name:
RSK3-pY217+pS218
Product Number:
ab-pk808
Target Full Name: Ribosomal S6 protein-serine kinase 3; Ribosomal protein S6 kinase alpha-3
Target Alias: 90 kDa ribosomal protein S6 kinase 2; HU-2; KS6A2; MAPKAPK1C; p90-RSK3; pp90RSK3; Ribosomal protein S6 kinase alpha 2; Ribosomal protein S6 kinase, 90kDa, polypeptide 2; RPS6KA2; S6K-alpha; S6K-alpha 2
Product Type Specific: RSK3 protein kinase phosphosite-specific antibody
Antibody Code: PK808
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y217+S218
Protein UniProt: Q15349
Protein SigNET: RSK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human RSK3 (RPS6KA2) sequence peptide Cat. No.: PE-04AHU95
Antibody Immunogen Sequence: KRA(pY)(pS)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K214 to G221; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. RSK3 S218 phosphorylation stimulates phosphotransferase activity and inhibits interaction with AKAP6. These are two of the major sites of in vivo phosphorylation of RSK2 based on ≥5 and ≥6 mass spectrometry reports recorded in PhosphoSitePlus. RSK3 is known to be phosphorylated in vitro at S218 by RSK3 (RPS6KA2).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse |Chicken | Frog | Zebra fish | Nematode worm.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-90 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities in Jurkat cells.
Related Product 1: RSK3-pY217+pS218 blocking peptide
Related Product 2: RSKSubtide - RSK1_d1 (RPS6KA2) protein kinase substrate peptide
Related Product 3: RPS6 (230-238) KinSub kinase substrate peptide (Cat. No.: PE-01ADQ95)
Related Product 4: RPS6 (230-239) KinSub kinase substrate peptide (Cat. No.: PE-01ADP95)
Related Product 5: RSK1 expression antibody (Cat. No.: AB-NK164-3)
Related Product 6: RSK1-pY220+pS221 phosphosite-specific antibody (Cat. No.: AB-PK807)
Related Product 7: RSK1 S221 phosphorylation antibody (Cat. No.: AB-PK804)
Related Product 8: RSK1 S380 phosphorylation antibody (Cat. No.: AB-PK805)
Related Product 9: RSK1 T573 phosphorylation antibody (Cat. No.: AB-PK806)
Related Product 10: RSK2 expression antibody (Cat. No.: AB-NK165-3)
Scientific Background: RSK3 (RPS6KA2) is a protein-serine/threonine kinase of the AGC group and RSK family. It is a protein-serine/threonine kinase member of the RSK (ribosomal S6 kinase) family of growth factor-regulated kinases. RSK proteins have two kinase catalytic domains and are phosphorylated by MAPK proteins in response to growth factors, polypeptide hormones, and neurotransmitters. RSK3 is activated by multiple phosphorylations on threonine and serine residues. Phosphorylation at S360 increases phosphotransferase activity. Phosphorylation at S218 inhibits interaction with AKAP6. RSK3 acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signalling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. It may function as tumour suppressor in epithelial ovarian cancer cells. This description may include information annotated by UniProt and/or Google AI.