Product Name:
RSK1-pS221
Product Number:
ab-pk804
Target Full Name: Ribosomal S6 protein-serine kinase 1; Ribosomal protein S6 kinase alpha 2
Target Alias: 90 kDa ribosomal protein S6 kinase 1; HU-1; Kinase p90RSK1; KS6A1; KS6AA; MAPKAPK1A; P90RSK1; RPS6KA1; S6K-alpha 1; CCDS284.1; ENSG00000117676
Product Type Specific: RSK1 protein kinase phosphosite-specific antibody
Antibody Code: PK804
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S221
Protein UniProt: Q15418
Protein SigNET: RSK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human RSK1 (RPS6KA1, p90RSK) sequence peptide Cat. No.: PE-04AGF99
Antibody Immunogen Sequence: KAY(pS)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K218 to G224; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S221 phosphorylation stimulates phosphotransferase activity. This is a major site of in vivo phosphorylation of RSK1 based on ≥133 high throughput mass spectrometry reports recorded in PhosphoSitePlus. RSK1 is known to be phosphorylated in vitro at S221 by PDPK1 (PDK1), and RSK1 (RPS6KA1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in HepG2 and Jurkat cells.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Nematode worm.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 82-92 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2 and Jurkat cells.
Related Product 1: RSK1-pS221 blocking peptide
Related Product 2: RSKSubtide - RSK1_d1 (RPS6KA2) protein kinase substrate peptide
Related Product 3: RPS6 (230-238) KinSub kinase substrate peptide (Cat. No.: PE-01ADQ95)
Related Product 4: RPS6 (230-239) KinSub kinase substrate peptide (Cat. No.: PE-01ADP95)
Related Product 5: RSK1 expression antibody (Cat. No.: AB-NK164-3)
Related Product 6: RSK1-pY220+pS221 phosphosite-specific antibody (Cat. No.: AB-PK807)
Related Product 7: RSK1 S380 phosphorylation antibody (Cat. No.: AB-PK805)
Related Product 8: RSK1 T573 phosphorylation antibody (Cat. No.: AB-PK806)
Related Product 9: RSK2 expression antibody (Cat. No.: AB-NK165-3)
Related Product 10: RSK3 Y217+S218 phosphorylation antibody (Cat. No.: AB-PK808)
Scientific Background: RSK1 (RPS6KA1) is a protein-serine/threonine kinase of the AGC group and RSK family, which features two kinase catalytic domains on the same polypeptide chain. This kinase is highly expressed and widely distributed in most tested human tissues. RSK1 transcript is present in lymphocytes, skeletal muscle, liver, and adipose tissue. RSK1 is activated by phosphorylation at S363, S380 and T573. Phosphorylation at S221 by PDK1 is required for increased phosphotransferase activity, but is insufficient by itself. Phosphorylation at S154 induces interaction with 14-3-3 and inhibits catalytic activity. RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation. RSK1 forms a complex with either ERK1 or ERK2 in quiescent cells, and phosphorylation of S732 in response to mitogenic stimulation of cells permits dissociation of RSK-ERK complexes. RSK1 is known phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway -- a pro-growth/anti-apoptotic pathway, as it will activate CREB, which in turn will upregulate expression of cyclin-D and c-Myc. Activation of this pathway is common in various types of tumours. RSK1 may be a tumour requiring protein (TRP). The active form of the protein kinase normally acts to promote tumour cell proliferation. Abnormal activation of RSK1 expression has been linked to many human diseases, including breast and prostate cancers. Inhibition of RSK signalling enhances the effects of enzalutamide prostate cancer therapy. This description may include information annotated by UniProt and/or Google AI.