Product Name:
Rock2-pY722
Product Number:
ab-pk799
Target Full Name: Rho-associated protein kinase 2
Target Alias: KIAA0619; Kinase Rock2; P164 Rock-2; RhoA-binding serine/threonine kinase alpha; Rho-associated protein kinase 2; Rho-associated, coiled-coil containing protein kinase 2; Rock-II; ROK-alpha
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK799
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y722
Protein UniProt: O75116
Protein SigNET: O75116
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Rock2 (ROKa) sequence peptide Cat. No.: PE-04ALB99
Antibody Immunogen Sequence: NKI(pY)ESI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N719 to I725; In the region between the kinase catalytic and Rho_Binding domains. The major site of phosphorylation in Rock2.
Production Method: Corresponds to amino acid residues N719 to I725; In the region between the kinase catalytic and Rho_Binding domains. The major site of phosphorylation in Rock2.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 160-180 kDa.
Antibody Specificity: Low-high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in Jurkat MCF7 cells. In HepG2 cells, a very high background was noted.
Related Product 1: Rock2-pY722 blocking peptide
Related Product 2: Rock1-pY913 phosphosite-specific antibody (Cat. No.: AB-PK798)
Related Product 3: Rock2Subtide - ROCK2 protein kinase substrate peptide
Scientific Background: Rock2 (ROK-alpha) is a protein-serine/threonine kinase of the AGC group and DMPK family. It regulates the actin cytoskeleton and cell polarity, thus is involved in the regulation of smooth muscle contraction, formation of stress fibre and focal adhesions, cell adhesion, and cell motility. It is activated by binding to the small G protein RhoA, and by phosphorylation at T967 and S1099. It is inhibited by phosphorylation at Y722. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
