Product Name:
Rock1-pY913
Product Number:
ab-pk798
Target Full Name: Rho-associated protein kinase 1
Target Alias: Kinase Rock1 ;p160Rock; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil forming protein kinase p160 Rock-1; Rock-I; ROK-beta
Product Type Specific: ROCK1 protein kinase phosphosite-specific antibody
Antibody Code: PK798
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y913
Protein UniProt: Q13464
Protein SigNET: Rock1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Rock1 (ROKb) sequence peptide Cat. No.: PE-04APL99
Antibody Immunogen Sequence: EEQ(pY)FEL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E910 to L916; Just before Rho-Binding domain. The effect of Y913 phosphorylation is unclear. This is the major site of in vivo phosphorylation of ROCK1 based on ≥104 high throughput mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in A431 cells, andMedium target protein detection in HepG2 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-170 kDa.
Antibody Specificity: Medium- very high
Antibody Cross Reactivity: In A431 cells, almost no significant cross-reactivities detected, except EGF decreases 150 kDa protein detection. In HepG2 cells, a few cross-reactive proteins are detected, including insulin increases in 75, 50, 37 and 15 kDa proteins.
Related Product 1: Rock1-pY913 blocking peptide
Related Product 2: Rock1Subtide - ROCK1 protein kinase substrate peptide
Related Product 3: ROCK1 T233 phosphorylation antibody (Cat. No.: AB-PK947)
Related Product 4: Rock2-pY722 phosphosite-specific antibody (Cat. No.: AB-PK799)
Scientific Background: ROCK1 (Rho-associated, coiled-coil-containing protein kinase 1; ROK-beta) is a 158. kDa protein-serine/threonine kinase of the AGC group and DMPK family. It is a key regulator of the actin cytoskeleton and cell polarity. It acts as a major downstream effector of the small GTPase RhoA, and is essential for regulating the actomyosin cytoskeleton, cell contractility, morphology, and motility. It is activated by binding the small G protein RhoA. ROCK1 regulates the cytoskeleton by phosphorylating targets like myosin light chain (MLC) and LIM kinase, which leads to increased contractility and stress fiber formation. It also plays a key role in metabolic stress-induced autophagy by phosphorylating Beclin1. ROCK1 features an N-terminus kinase domain and a C-terminus Rho-binding domain/pleckstrin homology domain. D1113A substitution leads to abolished cleavage by caspase-3. It is nvolved in cardiovascular diseases (e.g., hypertension), cancer metastasis, and fibrosis. Down-regulating ROCK1 may be useful strategy for inhibiting migration and invasion of glioma and gastric cancer.This description may include information annotated by UniProt and/or Google AI.