Product Name:
Rock1-pY913
Product Number:
ab-pk798
Target Full Name: Rho-associated protein kinase 1
Target Alias: Kinase Rock1 ;p160Rock; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil forming protein kinase p160 Rock-1; Rock-I; ROK-beta
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK798
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y913
Protein UniProt: Q13464
Protein SigNET: Q13464
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Rock1 (ROKb) sequence peptide Cat. No.: PE-04APL99
Antibody Immunogen Sequence: EEQ(pY)FEL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E910 to L916; Just before Rho-Binding domain. This is the major in vivo phosphorylation site in Rock1.
Production Method: Corresponds to amino acid residues E910 to L916; Just before Rho-Binding domain. This is the major in vivo phosphorylation site in Rock1.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in A431 cells, andMedium target protein detection in HepG2 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-170 kDa.
Antibody Specificity: Medium- very high
Antibody Cross Reactivity: In A431 cells, almost no significant cross-reactivities detected, except EGF decreases 150 kDa protein detection. In HepG2 cells, a few cross-reactive proteins are detected, including insulin increases in 75, 50, 37 and 15 kDa proteins.
Related Product 1: Rock1-pY913 blocking peptide
Related Product 2: Rock2-pY722 phosphosite-specific antibody (Cat. No.: AB-PK799)
Related Product 3: Rock1Subtide - ROCK1 protein kinase substrate peptide
Scientific Background: Rock1 (ROK-beta) is a protein-serine/threonine kinase of the AGC group and DMPK family. It is a key regulator of actin cytoskeleton and cell polarity. It is activated by binding the small G protein RhoA. D1113A substitution leads to abolished cleavage by caspase-3. Down-regulating Rock1 may be useful strategy for inhibiting migration and invasion of glioma and gastric cancer.
