Product Name:
RIPK2-pY381
Product Number:
ab-pk797
Target Full Name: Receptor-interacting serine/threonine-protein kinase 2 (RIPK2)
Target Alias: CARD3; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta converting enzyme associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARDIAK; GIG30; Kinase RIPK2; Receptor-interacting protein 2; Receptor-interacting serine/threonine protein kinase 2; RICK; RIP2; RIP-like-interacting CLARP kinase
Product Type Specific: RIPK2 protein kinase phosphosite-specific antibody
Antibody Code: PK797
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y381
Protein UniProt: O43353
Protein SigNET: RIPK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human RIPK2 (RIP2, RICK) sequence peptide Cat. No.: PE-04APJ90
Antibody Immunogen Sequence: QDA(pY)FMK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q378 to K384; in the region between the kinase catalytic and RHIM domains. Y381 phosphorylation stimulates phosphotransferase activity towards ARHGEF2. This is the major site of in vivo phosphorylation of RIPK2 based on ≥374 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-70 kDa.
Antibody Specificity: Very high
Related Product 1: RIPK2-pY381 blocking peptide
Related Product 2: RIPK1 Y384 phosphorylation antibody (Cat. No.: AB-PK795)
Related Product 3: RIPK1 S166 phosphorylation antibody (Cat. No.: AB-PK945)
Related Product 4: RIPK1 S320 phosphorylation antibody (Cat. No.: AB-PK946)
Related Product 5: RIPK2 S176 phosphorylation antibody (Cat. No.: AB-PK796)
Scientific Background: RIPK2 (Receptor-interacting serine/threonine-protein kinase 2; CARD3) is a 62 kDa protein-serine/threonine kinase of the TKL group and RIPK family. It plays an essential role in modulation of innate and adaptive immune responses. It bridges NOD1/NOD2 bacterial sensors to downstream pathways, activating NF-κB and MAPK to drive pro-inflammatory cytokine production. Upon bacterial peptidoglycan recognition, RIPK2 is recruited to NOD1/2 receptors and acts as a scaffold for TAK1 and IKK complexes, leading to cytokine release. RIPK2 features an N-terminal kinase domain and a C-terminal Caspase Activation and Recruitment Domain (CARD). It Is activated by phosphorylation at Ser-176. RIPK2 undergoes rapid lysine-63-linked polyubiquitination and auto-phosphorylation on serine/tyrosine residues (e.g., Tyr-474) upon activation. Dysregulation of RIPK2 is linked to inflammatory diseases (e.g., multiple sclerosis, IBD) and tumour development. This description may include information annotated by UniProt and/or Google AI.