Product Name:
RIPK2-pS176
Product Number:
ab-pk796
Target Full Name: Receptor-interacting serine/threonine-protein kinase 2 (RIPK2)
Target Alias: CARD3; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta converting enzyme associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARDIAK; GIG30; Kinase RIPK2; Receptor-interacting protein 2; Receptor-interacting serine/threonine protein kinase 2; RICK; RIP2; RIP-like-interacting CLARP kinase
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK796
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S176
Protein UniProt: O43353
Protein SigNET: O43353
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human RIPK2 (RIP2, RICK) sequence peptide Cat. No.: PE-04API75
Antibody Immunogen Sequence: MSL(pS)QSR(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M173 to R179; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues M173 to R179; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-70 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HEK-293, HeLa and T98G cells; in HEK-293 cells, phenylarsine oxide (PAO) decreases 28 kDa protein; in HeLa cells, phenylarsine oxide (PAO) increases 80 + 75 kDa proteins; in T98G cells, PDGF increases 27 + 22 kDa proteins.
Related Product 1: RIPK2-pS176 blocking peptide
Related Product 2: RIPK2-pY381 phosphosite-specific antibody (Cat. No.: AB-PK797)
Scientific Background: RIPK2 (CARD3) is a protein-serine/threonine kinase of the TKL group and RIPK family. It plays an essential role in modulation of innate and adaptive immune responses. It Is activated by phosphorylation at S176.
