Product Name:
RIOK1-pY466
Product Number:
ab-pk794
Target Full Name: Serine/threonine-protein kinase RIO1
Target Alias: AD034; BA288G3.1; FLJ30006; Kinase RIOK1; RIO kinase 1
Product Type Specific: RIOK1 protein kinase phosphosite-specific antibody
Antibody Code: PK794
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y466
Protein UniProt: Q9BRS2
Protein SigNET: RIOK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human RIOK1 sequence peptide Cat. No.: PE-04APK01
Antibody Immunogen Sequence: NIL(pY)QTV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N463 to V469; in the C-terminal third of the kinase after the catalytic domain. This is a major in vivo phosphorylation site in RIOK1. The effect of Y466 phosphorylation is unclear. This is a major in vivo phosphorylation site in RIOK1 based on ≥91 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 68-75 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in T98G cells.
Related Product 1: RIOK1-pY466 blocking peptide
Related Product 2: RIOK2 S332+S335+S337 phosphorylation antibody (Cat. No.: AB-PK890)
Scientific Background: RIOK1 (RIO Kinase 1) is a protein-serine/threonine kinase of the Atypical group and RIO family. It is an ATPase, essential for 40S ribosomal subunit maturation, cell growth, and division. It plays a crucial role in ribosome biogenesis by facilitating the final cytoplasmic maturation of 40S ribosomal subunits (18S-E pre-rRNA to 18S rRNA). It features a central RIO domain, and is localized primarily to the cytosol. It acts as an ATPase to facilitate the release of biogenesis factors and is considered an atypical kinase because it acts mostly as an ATPase and not a classical kinase. It acts as a substrate adaptor for the PRMT5 complex, involved in arginine methylation. RIOK1 mediates p53 degradation, thus promoting cancer cell survival, and it supports Akt signaling and mTOR activation, controlling cell growth. It localizes to the nucleus. It exists as two alternatively spliced isoforms. RIOK1 is frequently overexpressed in several human cancers (including breast, lung, and prostate) and is linked to high tumour grade and poor survival rates. This description may include information annotated by UniProt and/or Google AI.