Product Name:
Ret-pY905
Product Number:
ab-pk793
Target Full Name: Proto-oncogene tyrosine-protein kinase receptor Ret
Target Alias: CDHF12; C-ret; GDNF receptor; Glial cell line-derived neurotropic factor receptor; HSCR1; MEN2A; MEN2B; MTC1; PTC; Ret51; Ret-ELE1; CCDS7200.1; ENSG00000165731; B4DGX8; O43519; Q8IZR8; Q8NFE8; Q99886; Q9UM90; Q9UMQ4; F5CNE9; Q14277; Q15300; Q9UE13
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK793
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y905
Protein UniProt: P07949
Protein SigNET: P07949
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ret sequence peptide Cat. No.: PE-04AGL95
Antibody Immunogen Sequence: EDS(pY)VKR(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E902 to R908; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues E902 to R908; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 125-160 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2 and T98G cells.
Related Product 1: Ret-pY905 blocking peptide
Related Product 2: Ret-1 pan-specific antibody (Cat. No.: AB-NK244-1)
Related Product 3: Ret-2 pan-specific antibody (Cat. No.: AB-NK244-2)
Related Product 4: Ret-3 pan-specific antibody (Cat. No.: AB-NK244-3)
Related Product 5: IRS1 (891-902) KinSub - Insulin receptor substrate 1 (K891-G902) mouse, peptide; IGF1R protein kinase substrate peptide
Related Product 6: IRS1 (891-902) KinSub - Insulin receptor substrate 1 (K891-G902) mouse, peptide; IGF1Rtide protein kinase substrate peptide
Related Product 7: RetSubtide - Ret protein kinase substrate peptide
Scientific Background: Ret is a receptor protein-tyrosine in the TK group of protein kinases in the Ret family. It is highly expressed and widely distributed in most tested human tissues. Ret is activated by binding one of four structurally ligands (i.e. GDNF, neurturin, artemin and persephin). Autophosphorylation of S696, Y806, Y809, Y864, Y900, Y905, Y928, Y952, Y981 and Y1062 of Ret increases its phosphotransferase activity. Phosphorylation of Y1015 inhibits its phosphotransferase activity. Protein interactions are induced with phosphorylation of Y981 (with Src), Y1062 (with Crk, Dok6, FRS2, Gab1, IRS1, PIK3R1, RapGEF1, Shc1 & Shc3), and Y1096 (with Grb2 & PIK3R1). Phosphorylation of Y1015 inhibits kinase activity. Ret orchestrates intestine organogenesis. Germline mutations of Ret cause a dominantly inherited dysgenesis of the enteric nervous system known as Hirschsprung's disease. Ret can undergo oncogenic activation by cytogenetic rearrangement. It is constitutively activated by point mutations in hereditary medullary thyroid carcinomas (MTCs). Multiple endocrine neoplasia type 2A (MEN 2A) is associated with two mutations of Ret. It was identified as a potential low-penetrance gene for apparently sporadic pheochromocytoma. Ret has also been linked with congenital central hypoventilation syndrome, bladder carcinomas, and colorectal adenocarcinomas.
