Product Name:
PYK2-pY402
Product Number:
ab-pk788
Target Full Name: Protein tyrosine kinase 2 beta
Target Alias: CADTK; CAK beta; CAKB; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; FADK 2; FADK2, FAK2; FAK2; Related adhesion focal tyrosine kinase; RAFTK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK788
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y402
Protein UniProt: Q14289
Protein SigNET: Q14289
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PYK2 (PTK2B) sequence peptide Cat. No.: PE-04AQQ99
Antibody Immunogen Sequence: SDI(pY)AEI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S399 to I405; In the region between the FERM_M domain and the protein kinase catalytic domain
Production Method: Corresponds to amino acid residues S399 to I405; In the region between the FERM_M domain and the protein kinase catalytic domain
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-120 kDa.
Antibody Specificity: Very high
Related Product 1: PYK2-pY402 blocking peptide
Related Product 2: PYK2-pY402 phosphosite-specific antibody (Cat. No.: AB-PK787)
Related Product 3: PYK2-pY579+pY580 phosphosite-specific antibody (Cat. No.: AB-PK789)
Related Product 4: Pyk2Subtide - Pyk2 (PTK2B) protein kinase substrate peptide
Scientific Background: PYK2 (PTK2B, FAK2) is a protein-tyrosine kinase of the TK group and Fak family. It is a non-receptor kinase that has a role in actin cytoskeleton rearrangement, cell migration, cell polarization, adhesion, bone remodeling, and controlling amount of marginal B-cells' migration and presence in the spleen. Phosphorylation at TY402 increases phosphotransferase activity and induces interaction with ErbB2, RasGAP and Src. Phosphorylation at Y579 increases its phosphotransferase activity. NPHP1 interaction with PYK2 has been interrupted with a P859A mutation. A phosphorylation site of PYK2 can be inhibited with an Y881F mutation, which will also impair GRB2 interaction.
