Product Name:
PRP4K-pY849
Product Number:
ab-pk786
Target Full Name: Protein-serine kinase PRP4 homologue
Target Alias: CBP143; KIAA0536; Kinase PRP4; PR4H; Pre-mRNA protein kinase; PRPF4B; PRP4 homolog; PRP4 kinase; PRPK; PR4H; KIAA0536; dJ1013A10_1; PRP4H; PRP4K; ENSG00000112739
Product Type Specific: PRP4K protein kinase phosphosite-specific antibody
Antibody Code: PK786
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y849
Protein UniProt: Q13523
Protein SigNET: PRP4K
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PRP4K (PRP4, PRPF4B) sequence peptide Cat. No.: PE-04ADK99
Antibody Immunogen Sequence: ITP(pY)LVS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues I846 to S852. Y849 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major sites of in vivo phosphorylation of PRP4K based on ≥4107 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Sea urchin.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-100, 140 kDa.
Antibody Specificity: Very low
Related Product 1: PRPF4B (846-852) pY849 antibody blocking peptide (Cat. No.: PE-04ADK99)
Scientific Background: PRP4K (PRPF4B, PRP4) is a protein-serine/threonine kinase of the CMGC group and DYRK family. It is known to regulate pre-mRNA splicing. Identified substrates for PRP4K include splicing factors SF2, PRPF6, and PRPF31 as well as the KLF13 and Elk1 transcription factors. The enzymatic activity of PRP4K is required for viability of pancreatic, colon and large B-cell lymphoma cancer cell lines (PubMed: 24003220). Inhibition of PRP4K activity by shRNAs was shown to resensitize chemoresistant human ovarian and breast cancer to paclitaxel treatment. This description may include information annotated by UniProt and/or Google AI.