Product Name:
PRKX-p T201+pT203
Product Number:
ab-pk785
Target Full Name: Serine/threonine-protein kinase PRKX
Target Alias: EC 2.7.11.1; PKX1
Product Type Specific: PRKX protein kinase phosphosite-specific antibody
Antibody Code: PK785
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T201+T203
Protein UniProt: P51817
Protein SigNET: PRKX
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PRKX sequence peptide Cat. No.: PE-04APD99
Antibody Immunogen Sequence: VDR(pT)W(pT)LCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V198 to G206; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T201 and T203 phosphorylation are predicted to stimulate phosphotransferase activity. These are two of the major sites of in vivo phosphorylation of PRKX based on ≥12 and ≥28 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Fruit fly | Honey bee.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 38-44 kDa.
Related Product 1: PRKX (198-206) pT201+pT203 antibody blocking peptide (Cat. No.: PE-04APD99)
Related Product 2: PRKX expression antibody (Cat. No.: AB-NK292-1)
Related Product 3: PRKY expression antibody (Cat. No.: AB-NK293-1)
Scientific Background: PRKX (Protein Kinase X-linked; PKX1) is a protein-serine/threonine kinase of the AGC group and PKA family. It is an ancient, cAMP-dependent kinase that regulates crucial developmental processes, particularly renal epithelial morphogenesis and angiogenesis. Downstream targets of PRKX can include SMAD6, CREB, and PKD1. SMAD6 phosphorylation mediates differentiation of myeloid cells. It is expressed in various tissues, with the highest levels found in the fetal and adult brain, kidney, and lung. In the fetal kidney (specifically in the ureteric bud epithelium), PRKX acts to stimulate renal epithelial cell migration and branching morphogenesis. It stimulates crucial endothelial cell behaviours, including proliferation and the formation of vascular-like structures. While its catalytic core is highly conserved, its N-terminus is distinct from traditional PKA catalytic subunits. PRKX interacts with polycystin-1 and, when active, can rescue renal structural defects caused by PKD1 gene deficiency, indicating a role in mitigating Polycystic Kidney Disease (PKD) progression. It has been implicated in the tumorigenic process of nodular basal cell carcinoma and is part of the WNT signaling pathway. This description may include information annotated by UniProt and/or Google AI.