Product Name:
PRKD2-pS197+pS198
Product Number:
ab-pk784
Target Full Name: Serine/threonine-protein kinase D2
Target Alias: DKFZP586E0820; HSPC187; Kinase PKD2; KPCD2; KPCO; NPKC-D2; PRKD2; Protein kinase C, D2 type; Protein kinase D2 homolog
Product Type Specific: PKD2 protein kinase phosphosite-specific antibody
Antibody Code: PK784
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S197+S198
Protein UniProt: Q9BZL6
Protein SigNET: PRKD2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PRKD2 (PKD2) sequence peptide Cat. No.: PE-04APB99
Antibody Immunogen Sequence: RRL(pS)(pS)TSL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R194 to L201; in the C1_1 domain. The effects of S197 and S198 phosphorylation are unclear. These are the major in vivo sites of phosphorylation of PRKD2 (≥353 and ≥190 reports, respectively, by mass spectrometry).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-105 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in T98G cells.
Scientific Background: PRKD2 (PKD2) is a protein-serine/threonine kinase of the CAMK group and PKD family. It lengthens the signal induced by diacylglycerol (DAG) by modulating the cascade downstream from PKC. It is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating PRKD2 translocation to membranes. Autophosphorylation of S710 and phosphorylation of S706 by PKC relieves auto-inhibition by the PH domain PRKD2 can mediate NF-kB activation (induced from oxidative stress), cell proliferation (induced from MAPK1/3 signalling), inhibition of the transcription repression for HDAC7, T-cell antigen receptor signalling cascade, golgi apparatus vesicle trafficking, cell adhesion, angiogenesis, and secretory granule release. This description may include information annotated by UniProt and/or Google AI.