Product Name:
PRKACA-pT196+pT198
Product Number:
ab-pk782
Target Full Name: cAMP-dependent protein kinase catalytic subunit alpha
Target Alias: Alpha-catalytic subunit; cAMP-dependent protein kinase; cAMP-dependent protein kinase, alpha-catalytic subunit; cAPKa; KAPCA; Kinase PKA C-alpha; PKA C-alpha; PKA-alpha; PKACA; Protein kinase, cAMP-dependent, catalytic, alpha; MGC102831; MGC48865; CCDS12304.1; P17612; ENSG00000072062
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK782
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T196+T198
Protein UniProt: P17612
Protein SigNET: P17612
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PRKACA sequence peptide Cat. No.: PE-04AOQ85
Antibody Immunogen Sequence: KGR(pT)W(pT)LCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K193 to G201; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues K193 to G201; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 38-42 kDa.
Related Product 1: PRKACA-pT196+pT198 blocking peptide
Related Product 2: CREB1 (123-135) KinSub - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Related Product 3: CREB1 (123-135) KinSub, biotinyl. - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Related Product 4: KEMPtide KinSub - PKA protein kinase substrate peptide
Related Product 5: PKASubtide - PKACb (PRKACB) protein kinase substrate peptide
Scientific Background: PKACa (PRKACA) is a protein-serine/threonine kinase of the AGC group and PKA family. It serves as the alpha catalytic subunit of the cAMP-dependent protein kinase holoenzyme PKA, and it phosphorylates and regulates a very large number of cytoplasmic and nuclear substrate proteins. Phosphorylates hundreds of substrates in the cytoplasm and the nucleus. These include CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, TRPC1 and VASP. It is activated by binding of two cAMP molecules to each of the two associated regulatory subunits in the PKA holoenzyme. Binding of cAMP induces dissociation of the two active catalytic subunits. Phosphorylation of T198 increases phosphotransferase activity. Phosphorylation of S338 plays a key role in stabilizing PKACa. PKACa may be an oncoprotein (OP), although this kinase has also been implicated in inhibiting normal cell proliferation. Gain-of-function mutations in the PRKACA catalytic domain are associated with elevated catalytic activity of the kinase, and have been observed in several cancer types. In particular, a L206R subsitution mutation is observed in cancer and in Cushing's syndrome patients. The gain-of-function mutation is predicted to result in the interruption of the interaction between the catalytic and regulatory subunits of protein kinase A, resulting in a constitutively active PKA. Gain-of-function mutations in the PRKACA gene that lead to consitutive activity of the PKA holoenzyme have been proposed to play an important role in the development of several types of cancer.
