Product Name:
PRK1-pT774
Product Number:
ab-pk781
Target Full Name: Protein kinase C-related protein-serine kinase 1; Protein kinase N1
Target Alias: PAK-1; PKN; PRK1; PRKCL1; Protease-activated kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein-kinase C-related kinase 1
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK781
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T774
Protein UniProt: Q16512
Protein SigNET: Q16512
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PRK1 (PKN1) sequence peptide Cat. No.: PE-04ADX99
Antibody Immunogen Sequence: RTS(pT)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R771 to G777; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues R771 to G777; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 125-130 kDa.
Antibody Specificity: Very high
Related Product 1: PRK1-pT774 blocking peptide
Scientific Background: PRK1 (PKN1) is a protein-serine/threonine kinase of the AGC group and PKN family. It is associated with many functions including regulation of actin cytoskeleton, cell migration, and tumour cell invastion. Its phosphotransferase activity is stimulated upon binding to Rho proteins (RHOA, RHOB and RAC1). It is activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. It is also activated by caspase-3 (CASP3) cleavage during apoptosis., as well as in vitro by limited proteolysis by trypsin. Phosphorylationof PRK1 at T774 (activation loop of the kinase domain) and S916 (turn motif) increases phosphotransferase activity. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. Stimulation of the PRK1 signalling cascade results in androgen receptor superactivation in human prostate cancer cells. PRK1 also represses Wnt signalling in human melanoma cells.
