Product Name:
PRK1-pT774
Product Number:
ab-pk781
Target Full Name: Protein kinase C-related protein-serine kinase 1; Protein kinase N1
Target Alias: PAK-1; PKN; PRK1; PRKCL1; Protease-activated kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein-kinase C-related kinase 1
Product Type Specific: PKN1 protein kinase phosphosite-specific antibody
Antibody Code: PK781
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T774
Protein UniProt: Q16512
Protein SigNET: PRK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PRK1 (PKN1) sequence peptide Cat. No.: PE-04ADX99
Antibody Immunogen Sequence: RTS(pT)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R771 to G777. T774 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. Phosphorylation regulates cytoskeletal reorganization and molecular association. PKN1 is known to be phosphorylated at this site in vitro by PDPK1 (PDK1), and PRK1 (PKN1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Zebra fish | Nematode worm | Sea urchin.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 125-130 kDa.
Antibody Specificity: Very high
Related Product 1: PKN1 (771-777) pT774 antibody blocking peptide (Cat. No.: PE-04ADX99)
Scientific Background: PRK1 (PKN1) is a protein-serine/threonine kinase of the AGC group and PKN family. It is associated with many functions including regulation of actin cytoskeleton, cell migration, and tumour cell invastion. Its phosphotransferase activity is stimulated upon binding to Rho proteins (RHOA, RHOB and RAC1). It is activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. It is also activated by caspase-3 (CASP3) cleavage during apoptosis., as well as in vitro by limited proteolysis by trypsin. Phosphorylationof PRK1 at T774 (activation loop of the kinase domain) and S916 (turn motif) increases phosphotransferase activity. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. Stimulation of the PRK1 signalling cascade results in androgen receptor superactivation in human prostate cancer cells. PRK1 also represses Wnt signalling in human melanoma cells. This description may include information annotated by UniProt and/or Google AI.