Product Name:
Plk4-pT170
Product Number:
ab-pk780
Target Full Name: Polo-like kinase-serine 4 (SAK)
Target Alias: Plk-4; Polo-like kinase 4; Sak; Serine,threonine-protein kinase 18; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; STK18; CCDS3735.1; O00444; ENSG00000142731
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK780
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T170
Protein UniProt: O00444
Protein SigNET: O00444
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Plk4 (SAK; STK18) sequence peptide Cat. No.: PE-04AKZ99
Antibody Immunogen Sequence: KHY(pT)LCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K167 to G173; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues K167 to G173; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 / 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: Plk4-pT170 blocking peptide
Related Product 2: Plk4-MID pan-specific antibody (Cat. No.: AB-NK291-1)
Scientific Background: Plk4 (SAK) is a protein-serine/threonine kinase of the Other group and Plk family. It has a key function in centriole duplication through the recruitment of the centriole biogenesis proteins SASS6, CENPJ/CPAP, CCP110, CEP135, and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. It phosphorylates S151 of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. It is involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activation of HAND1. It also phosphorylates CDC25C and Chk2 (CHEK2). Its central role in centriole replication indicates that it may have a possible role in tumourigenesis, as centrosome aberrations are frequently observed in tumours.
