Product Name:
PKG1-pT515+pT517
Product Number:
ab-pk776
Target Full Name: cGMP-dependent protein kinase 1, alpha isozyme
Target Alias: cGK 1 alpha; cGKI-alpha; cGKI-beta; cGMP-dependent protein kinase 1, alpha isozyme; DKFZp686K042; FLJ36117; KGP1A; Kinase PKG1; PGK; PKG; PKG1-alpha; Protein kinase, cGMP-dependent, type I
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK776
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T515+T517
Protein UniProt: Q13976
Protein SigNET: Q13976
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKG1a (PRKG1A) sequence peptide Cat. No.: PE-04ARO99
Antibody Immunogen Sequence: FGKK(pT)W(pT)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F511 to G521; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues F511 to G521; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 77-83 kDa.
Related Product 1: PKG1-pT515+pT517 blocking peptide
Related Product 2: PKG1-BKCD pan-specific antibody (Cat. No.: AB-NK202-2)
Related Product 3: PKG1a-NT (PKG1a-1) pan-specific antibody (Cat. No.: AB-NK202)
Related Product 4: PKG1b-NT (PKG1b-1) pan-specific antibody (Cat. No.: AB-NK203)
Related Product 5: PKG1Subtide - PKG1 (PRKG1) protein kinase substrate peptide
Scientific Background: PKG1 (PRKG1) is a protein-serine/threonine kinase of the AGC group and PKG family. It is activated by cGMP, which is generated in response to nitric oxide. Phosphorylation at T59 increases phosphotransferase activity and its affinity for cGMP Phosphorylation at T517 increases phosphotransferase activity. An R177Q mutation in AAT8 results in a constitutively active form of PKG1, which also has reduced cGMP binding.
