Product Name:
PKCd-pY334
Product Number:
ab-pk769
Target Full Name: Protein-serine kinase C delta
Target Alias: Kinase PKC-delta; KPCD; MAY1; MGC49908; nPKC-delta; PKC-delta; PRKCD; Protein kinase C, delta; CCDS2870.1; ENSG00000163932
Product Type Specific: PKCd protein kinase phosphosite-specific antibody
Antibody Code: PK769
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y334
Protein UniProt: Q05655
Protein SigNET: PKCd
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKCd (PRKCD) sequence peptide Cat. No.: PE-04AOW95
Antibody Immunogen Sequence: SGT(pY)GKI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S331 to I337; in the region between the C1_1 and kinase catalytic domain. Y334 phosphorylation stimulates phosphotransferase activity and regulates apoptosis and molecular association, including inducing interaction with Src. This is one of the major in vivo phosphorylation sites in PKCd (≥545 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PKCd is known to be phosphorylated at this site in vitro by EGFR, Fyn, Lck, Lyn, PDGFRA, PDGFRB, PKCd (PRKCD), Src, and Yes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.0 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 78-85 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: PKCd (331-337) pY334 antibody blocking peptide (Cat. No.: PE-04AOW95)
Related Product 2: CREB1 (123-135) KinSub kinase substrate peptide (Cat. No.: PE-01ACP95)
Related Product 3: PKCA/BSubtide kinase substrate peptide (Cat. No.: PE-01BIR95)
Related Product 4: PKCtide KinSub kinase substrate peptide (Cat. No.: PE-01ADL95)
Related Product 5: Syntide-2 KinSub kinase substrate peptide (Cat. No.: PE-01AQB95)
Related Product 6: PKCd T507 phosphorylation antibody (Cat. No.: AB-PK767)
Related Product 7: PKCd Y313 phosphorylation antibody (Cat. No.: AB-PK768)
Related Product 8: PKCq S662 phosphorylation antibody (Cat. No.: AB-PK971)
Related Product 9: PKCq S685 phosphorylation antibody (Cat. No.: AB-PK961)
Related Product 10: PKCz T410 phosphorylation antibody (Cat. No.: AB-PK775)
Scientific Background: PKCd (PRKCD, PKC-delta) is a protein-serine/threonine kinase in the novel protein kinase C family. It is dependent on acidic phospholipids (e.g. phosphatidylserine) and diacylglycerol for full phosphotransferase activity, and does not require calcium. It is highly expressed and widely distributed in most tested human tissues, and is a major isoform of PKC expressed in hemopoietic cells. It is involved in promoting apoptosis, differentiation of cell types, cell growth, regulation of transcription factors, NFkB signalling induced by reactive oxygen species, and antigen presentation. PKCd is activated by phosphorylation at Y313, Y514, Y525, and S645. Phosphorylation of T507 increases phosphotransferase activity and induces degradation of PKC-delta. Phosphorylation of Y52, Y64, Y187, and Y567 inhibits phosphotransferase activity. Phosphatidylinositol is the most potent activator of PKCd. PKC delta is involved in fundamental cellular functions regulated by diacylglycerols, which is mimicked by phorbol esters. PKCd may be a tumour suppressor protein (TSP). PKCd negatively regulates cell proliferation in colon cell lines. It is also required for ErbB2-driven mammary gland tumorigenesis. Overexpression is seen in breast cancer and is associated with tamoxifen resistance. Reduced expression in MCF-7 cells is associated with a metastatic phenotype. This description may include information annotated by UniProt and/or Google AI.