Product Name:
PIP5K-pS307
Product Number:
ab-pk762
Target Full Name: 1-phosphatidylinositol-3-phosphate 5-kinase
Target Alias: 1- phosphatidylinositol-4-phosphate 5-kinase; EC 2.7.1.68; FYV1; FYVE finger-containing phosphoinositide kinase; FYVE finger-containing PI kinase; KIAA0981; Kiaa0981; MGC40423; P235; Phosphoinositide kinase, FYVE finger containing; PIKfyve; PIP5K3; PtdIns(4)P-5- kinase; PtdIns(4)P-5-kinase
Product Type Specific: PIP5K protein kinase phosphosite-specific antibody
Antibody Code: PK762
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S307
Protein UniProt: Q9Y2I7
Protein SigNET: PIP5K
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PIP5K sequence peptide Cat. No.: PE-04AOO99
Antibody Immunogen Sequence: RSA(pS)ITN(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R304 to N310; in the region between the FYVE and DEP domains in the N-terminal part of the kinase.S307 phosphorylation stimulates the ability of PIKFYVE to catalyze the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate. This is one of the major in vivo phosphorylation sites in PIP5K (≥199 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PIP5K is known to be phosphorylated at this site in vitro by Akt1 (PKBa), and AMPKa1 (PRKAA1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 230-275 kDa.
Related Product 1: PIP5K (304-310) pS307 antibody blocking peptide (Cat. No.: PE-04AOO99)
Scientific Background: PIP5K (PIKFYVE) is a protein-serine/threonine kinase of the Atypical group. It regulates the production and degradation of phosphatidylinositol 3,5-bisphosphate. The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). It catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. It is required for endocytic-vacuolar pathway and nuclear migration, and plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes. A PIP5K inhibitor, YM201636, significantly increased the survival of C9ORF72 patient- derived motor neurons by converting PtdIns3P into PtdIns(3,5)P2, which enhanced the fusion of lysosomes with both endosomes and autophagosomes under cell stress (1). This description may include information annotated by UniProt and/or Google AI.