Product Name:
Pim2-pT195
Product Number:
ab-pk761
Target Full Name: Serine/threonine-protein kinase Pim-2
Target Alias: Pim-2h; ENSG00000102096
Product Type Specific: Pim2 protein kinase phosphosite-specific antibody
Antibody Code: PK761
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T195
Protein UniProt: Q9P1W9
Protein SigNET: Pim2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Pim2 sequence peptide Cat. No.: PE-04AOP95
Antibody Immunogen Sequence: EPY(pT)DFD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E192 to D198; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T195 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This is one of the few in vivo phosphorylation sites in Pim2 (≥1 report from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 34-38 kDa.
Related Product 1: PIM2 (192-198) pT195 antibody blocking peptide (Cat. No.: PE-04AOP95)
Related Product 2: PimSelectide - Pim1 protein kinase substrate peptide
Related Product 3: RPS6 (230-238) KinSub kinase substrate peptide (Cat. No.: PE-01ADQ95)
Related Product 4: Pim1 expression antibody (Cat. No.: AB-NK258-2)
Related Product 5: Pim2 expression antibody (Cat. No.: AB-NK288-1)
Related Product 6: Pim2 expression antibody (Cat. No.: AB-NK288-2)
Related Product 7: Pim3 expression antibody (Cat. No.: AB-NK289-1)
Scientific Background: Pim2 is a protein-serine/threonine kinase of the CAMK group and Pim family. The Pim2 protein, along with the related Pim1 and Pim3 proteins, promotes cell survival and proliferation by acting downstream of cytokine and growth factor signalling pathways. It regulates Myc transcription and stabilization by phosphorylation. It also prevents apoptosis by phosphorylating and inactivating the proapoptotic protein Bad. It induces cell survival and proliferation signals via NF-KB cascade and phosphorylation and inhibition of CDKN1A and CDKN1B. Dysregulation of Pim2 can promote cell proliferation and resistance to apoptosis and oncogenesis. The very low frequency of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours. Although it is considered as an oncoprotein (OP), it shows a level of bystander mutations typical of most proteins in human cancers. The active form of the protein kinase normally acts to promote tumour cell proliferation. Pim2 is linked to Mantle Cell Lymphomas (LCM), which are non-Hodgkin lymphomas in which is specifically a B-cell lymphoma is also associated the ATM (ataxia telangiectasia mutated) gene. This description may include information annotated by UniProt and/or Google AI.