Product Name:
Pim2-pT195
Product Number:
ab-pk761
Target Full Name: Serine/threonine-protein kinase Pim-2
Target Alias: Pim-2h; ENSG00000102096
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK761
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T195
Protein UniProt: Q9P1W9
Protein SigNET: Q9P1W9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Pim2 sequence peptide Cat. No.: PE-04AOP95
Antibody Immunogen Sequence: EPY(pT)DFD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E192 to D198; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues E192 to D198; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 34-38 kDa.
Related Product 1: Pim2-pT195 blocking peptide
Related Product 2: Pim2-BKCD pan-specific antibody (Cat. No.: AB-NK288-1)
Related Product 3: Pim2-CT pan-specific antibody (Cat. No.: AB-NK288-2)
Related Product 4: PimSelectide - Pim1 protein kinase substrate peptide
Related Product 5: RPS6 (230-238) KinSub - 40S ribosomal protein S6 (K230-R238, human) peptide; p70S6K protein kinase substrate peptide
Scientific Background: Pim2 is a protein-serine/threonine kinase of the CAMK group and Pim family. The Pim2 protein, along with the related Pim1 and Pim3 proteins, promotes cell survival and proliferation by acting downstream of cytokine and growth factor signalling pathways. It regulates Myc transcription and stabilization by phosphorylation. It also prevents apoptosis by phosphorylating and inactivating the proapoptotic protein Bad. It induces cell survival and proliferation signals via NF-KB cascade and phosphorylation and inhibition of CDKN1A and CDKN1B. Dysregulation of Pim2 can promote cell proliferation and resistance to apoptosis and oncogenesis. The very low frequency of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours. Although it is considered as an oncoprotein (OP), it shows a level of bystander mutations typical of most proteins in human cancers. The active form of the protein kinase normally acts to promote tumour cell proliferation. Pim2 is linked to Mantle Cell Lymphomas (LCM), which are non-Hodgkin lymphomas in which is specifically a B-cell lymphoma is also associated the ATM (ataxia telangiectasia mutated) gene.
