Product Name:
PDK1-pS241
Product Number:
ab-pk760
Target Full Name: 3-phosphoinositide-dependent protein-serine kinase 1
Target Alias: 3-phosphoinositide dependent protein kinase-1; HPDK1; Kinase PDPK1; MGC20087; MGC35290; MPDK1; PDPK1; PkB kinase; PkB-like; PkB-like 2; Protein kinase B kinase
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK760
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S241
Protein UniProt: O15530
Protein SigNET: O15530
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PDK1 (PDPK1) sequence peptide Cat. No.: PE-04AKW99
Antibody Immunogen Sequence: RAN(pS)FVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R238 to G244; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues R238 to G244; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-65 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2 and HeLa cells; in HeLa cells, phenylarsine oxide (PAO) increases 25 kDa protein.
Related Product 1: PDK1-pS241 blocking peptide
Related Product 2: PDK1-NT pan-specific antibody (Cat. No.: AB-NK126-4)
Related Product 3: PDKSubtide - PDK1 (PDPK1) protein kinase substrate peptide
Related Product 4: PDKtide KinSub - PDKtide peptide substrate
Scientific Background: PDK1 (PDPK1) is a protein-serine/threonine kinase of the AGC group and PKB family. It activates, via phosphorylation, many members of the AGC family of protein kinases. It is activated by phosphorylaton at Y9, S160, S241, Y373, and Y376. MELK-mediated phosphorylation can be inhibited through a T354A mutation. PDK1 phosphorylation of MAP3K5 can be ablated through the S394A and S398A mutations. PKB has been observed to be phosphorylated more via a T513E mutation in PDK1. Its phosphotransferase activity can be reduced 3-fold with a A277V mutation. PDK1 is important in mediating migration and metastasis of breast cancer cells.
