Product Name:
PDK1-pS241
Product Number:
ab-pk760
Target Full Name: 3-phosphoinositide-dependent protein-serine kinase 1
Target Alias: 3-phosphoinositide dependent protein kinase-1; HPDK1; Kinase PDPK1; MGC20087; MGC35290; MPDK1; PDPK1; PkB kinase; PkB-like; PkB-like 2; Protein kinase B kinase
Product Type Specific: PDK1 protein kinase phosphosite-specific antibody
Antibody Code: PK760
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S241
Protein UniProt: O15530
Protein SigNET: PDK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PDK1 (PDPK1) sequence peptide Cat. No.: PE-04AKW99
Antibody Immunogen Sequence: RAN(pS)FVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R238 to G244; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S241 phosphorylation is stimulatory for phosphotransferase activity. Phosphorylation induces interaction with 14-3-3 eta and 14-3-3 theta. This is the major in vivo phosphorylation site in PDK1 (≥334 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PDK1 is known to be phosphorylated at this site in vitro by PDPK1 (PDK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-65 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2 and HeLa cells; in HeLa cells, phenylarsine oxide (PAO) increases 25 kDa protein.
Related Product 1: PDK1 (238-244) pS241 antibody blocking peptide (Cat. No.: PE-04AKW99)
Related Product 2: PDHKSubtide kinase substrate peptide (Cat. No.: PE-01BIO90)
Related Product 3: PDK1-NT pan-specific antibody (Cat. No.: AB-NK126-4)
Related Product 4: PDK1-CT pan-specific antibody (Cat. No.: AB-NK126-3)
Scientific Background: PDK1 (PDPK1) is a protein-serine/threonine kinase of the AGC group and PKB family. It features a catalytic kinase domain and a C-terminal pleckstrin homology (PH) domain. PDK1 activates, via phosphorylation, many members of the AGC family of protein kinases (e.g., Akt/PKB, PKC, S6K, and SGK). It regulates cell growth, survival, and proliferation. It is recruited to the plasma membrane by binding to phospholipids like phosphatidylinositol-(3,4,5)-trisphosphate (PIP3), which allows it to phosphorylate and activate substrates. PDK1 is activated by phosphorylaton at Tyr-9, Ser-160, Ser-241, Tyr-373, and Tyr-376. MELK-mediated phosphorylation can be inhibited through a T354A mutation. PDK1 phosphorylation of MAP3K5 can be ablated through the S394A and S398A mutations. PKB has been observed to be phosphorylated more via a T513E mutation in PDK1. Its phosphotransferase activity can be reduced 3-fold with a A277V mutation. PDK1 is important in mediating migration and metastasis of breast cancer cells. This description may include information annotated by UniProt and/or Google AI.