Product Name:
PCTK2-pS180
Product Number:
ab-pk756
Target Full Name: Cell division protein kinase 17; Protein-serine kinase PCTAIRE-2
Target Alias: CDK17; KPT2; PCTAIRE protein kinase 2; PCTAIRE-motif protein kinase 2; PCTK2; Serine/threonine-protein kinase PCTAIRE-2
Product Type Specific: PCTAIRE2 (CDK17) protein kinase phosphosite-specific antibody
Antibody Code: PK756
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S180
Protein UniProt: Q00537
Protein SigNET: PCTK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCTK2 (PCTAIRE2, CDK17)) sequence peptide Cat. No.: PE-04AJM99
Antibody Immunogen Sequence: RRA(pS)LSE(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R177 to E183; Just before kinase catalytic domain. One of two major sites of phosphorylation of PCTK2. The effect of S180 phosphorylation is unclear. One of two major sites of phosphorylation of PCTK2 (≥342 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-65 kDa.
Antibody Specificity: Very high
Related Product 1: PCTK2-ANT pan-specific antibody (Cat. No.: AB-NK285-1)
Related Product 2: PCTK1 Y176 phosphorylation antibody (Cat. No.: AB-PK755)
Related Product 3: PCTK3 expression antibody (Cat. No.: AB-NK286-1)
Scientific Background: PCTK2 (PCTAIRE2, CDK17) is a protein-serine/threonine kinase of the CMGC group and CDK family. It is mainly expressed in terminally differentiated neurons and plays a key role in neuronal function. PCTK2 possesses a central kinase domain that is highly conserved (~80% identity) with other PCTAIRE isoforms, along with unique N-terminal and C-terminal domains. It is associated with cyclin Y. It is also identified to be associated with circulating phospholipid and sphingolipid concentrations. This kinase likely phosphorylates histone H1. Insertional mutagenesis studies in mice support a role for PCTK2 in mouse cancer oncogenesis. This description may include information annotated by UniProt and/or Google AI.