Product Name:
PCTK1-pY176
Product Number:
ab-pk755
Target Full Name: Cell division protein kinase 16; Protein-serine kinase PCTAIRE-1
Target Alias: CDK16; FLJ16665; KPT1; PCTAIRE; PCTAIRE protein kinase 1; PCTAIRE-motif protein kinase 1; PCTGAIRE; PCTK1; Serine/threonine-protein kinase PCTAIRE1; ENSG00000102225
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK755
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y176
Protein UniProt: Q00536
Protein SigNET: Q00536
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCTK1 (PCTAIRE1, CDK16) sequence peptide Cat. No.: PE-04AJL85
Antibody Immunogen Sequence: EGT(pY)ATV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E173 to V179; In the protein kinase catalytic domain in subdomain I. This is the major in vivo phosphorylation site in PCTK1.
Production Method: Corresponds to amino acid residues E173 to V179; In the protein kinase catalytic domain in subdomain I. This is the major in vivo phosphorylation site in PCTK1.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in HeLa cells that are treated for 30 minutes with phenylarsine oxide and vanadate. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-64 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: In HeLa cells, phenylarsine oxide (PAO) increases detection of 100, 80, 75, 56, 48 and 27 kDa proteins.
Related Product 1: PCTK1-pY176 blocking peptide
Scientific Background: PCTK1 (PCTAIRE1, CDK16) is a protein-serine/threonine kinase of the CMGC group and CDK family. It has an important role in vesicle trafficking. Specifically neuronal release of GH1 is mediated by PCTK1. It is also involved in spermatogenesis, dendrite development, neuronal differentiation, and insulin secretion. BRSK2 mediated phosphorylation of PCTK1 can be inhibited with aPCTK1S12A mutation, which leads to fully inhibited effect on insulin secretion. The interaction of PCTK1 and CCNY can be inhibited with a S119 phosphorylation and PCTK1 phosphotransferase activity can be constitutively activated through the mutation S153A. Interaction with CCNY can be inhibited with a S153D mutation. Phosphorylation at S119 induces interaction with 14-3-3 gamma and 14-3-3 zeta. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
