Product Name:
p70S6K-pT412
Product Number:
ab-pk745
Target Full Name: Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase 1
Target Alias: RPS6KB1; KS6B1; Ps6K; p70(S6K)-alpha; Ribosomal protein S6 kinase I; RPS6KB1; S6K; S6K1; STK14A
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK745
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T412
Protein UniProt: P23443
Protein SigNET: P23443
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human p70S6K (S6Ka, RPS6KB1) sequence peptide Cat. No.: PE-04AGH85
Antibody Immunogen Sequence: LGF(pT)YVA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L409 to A415; In the C-terminal third of the kinase
Production Method: Corresponds to amino acid residues L409 to A415; In the C-terminal third of the kinase
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 63-70 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in Jurkat cells, phenylarsine oxide (PAO) decreases 75 + 90 kDa proteins, and phenylarsine oxide (PAO) increases 60 kDa protein.
Related Product 1: p70S6K-pT412 blocking peptide
Related Product 2: p70S6K-pT252 phosphosite-specific antibody (Cat. No.: AB-PK744)
Related Product 3: p70S6K-pT444+pS447 phosphosite-specific antibody (Cat. No.: AB-PK746)
Related Product 4: RPS6 (230-238) KinSub - 40S ribosomal protein S6 (K230-R238, human) peptide; p70S6K protein kinase substrate peptide
Related Product 5: S6KASubtide - p70S6K (RPS6KB1) protein kinase substrate peptide
Related Product 6: GSK3b (3-12) KinSub - Glycogen synthase kinase 3-beta (GSK3b) N-terminus (G3-E12, human) peptide; Akt protein kinase substrate peptide
Scientific Background: p70S6K (RPS6KB1) is a protein-serine/threonine kinase that is a member of the AGC group of protein kinases in the RSK family, and p70 subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord where it is more moderately expressed. It functions downstream of mTOR (FRAP1) signalling in response to growth factors to promote cell proliferation, growth, and progression through the cell cycle. p70S6K is activated by phosphorylation at T252 (by PDK1), S394 (by mTOR), T412 (by mTOR), S427, S434 (by ERK1/2), S441, T444 (by ERK1/2) and S447 (by ERK1/2). p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6. It is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K phosphotransferase activity changes during the cell cycle, and increases 20-fold in G1 cells released from G0. It has been linked with the development of diet-induced obesity, colon adenocarcinoma and breast cancer.
