Product Name:
p70S6K-pT252
Product Number:
ab-pk744
Target Full Name: Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase 1
Target Alias: RPS6KB1; KS6B1; Ps6K; p70(S6K)-alpha; Ribosomal protein S6 kinase I; RPS6KB1; S6K; S6K1; STK14A
Product Type Specific: p70S6K protein kinase phosphosite-specific antibody
Antibody Code: PK744
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T252
Protein UniProt: P23443
Protein SigNET: p70S6K
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human p70S6K (S6Ka, RPS6KB1) sequence peptide Cat. No.: PE-04AKT99
Antibody Immunogen Sequence: VTH(pT)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V249 to G255. T252 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. Phosphorylation regulates protein translation and protein conformation. This is one of the minor in vivo phosphorylation sites in p70 S6K (≥18 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). p70S6K is known to be phosphorylated at this site in vitro by PDPK1 (PDK1), PIK3CA, and PIK3CD.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Sea urchin.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 63-70 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2 and T98G cells, and in HeLa cells, phenylarsine oxide (PAO) decreases detection of 260 + 75 kDa proteins.
Related Product 1: p70S6K (249-255) pT252 antibody blocking peptide (Cat. No.: PE-04AKT99)
Related Product 2: RPS6 (230-238) KinSub kinase substrate peptide (Cat. No.: PE-01ADQ95)
Related Product 3: S6KASubtide kinase substrate peptide (Cat. No.: PE-01BJH95)
Related Product 4: GSK3b (3-12) KinSub - Glycogen synthase kinase 3-beta (GSK3b) N-terminus (G3-E12, human) peptide; Akt protein kinase substrate peptide
Related Product 5: p70S6K T412 phosphorylation antibody (Cat. No.: AB-PK745)
Related Product 6: p70S6K T444+S447 phosphorylation antibody (Cat. No.: AB-PK746)
Related Product 7: p70S6KB S423 phosphorylation antibody (Cat. No.: AB-PK747)
Scientific Background: p70S6K (RPS6KB1) is a protein-serine/threonine kinase that is a member of the AGC group of protein kinases in the RSK family, and p70 subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord where it is more moderately expressed. It functions downstream of mTOR (FRAP1) signalling in response to growth factors to promote cell proliferation, growth, and progression through the cell cycle. p70S6K is activated by phosphorylation at Thr-252 (by PDK1), Ser-394 (by mTOR), Thr-412 (by mTOR), Ser-427, Ser-434 (by ERK1/2), Ser-441, Thr-444 (by ERK1/2) and Ser-447 (by ERK1/2). It plays a crucial role in promoting protein synthesis, cell growth, and proliferation. Acting downstream of the mTORC1 signaling pathway, it phosphorylates the S6 ribosomal protein, which stimulates ribosome function and translation, particularly in response to growth factors and nutrients. It also targets components such as EIF4B, and EEF2K to boost protein synthesis. Its activation by serum stimulation is inhibited by wortmannin and rapamycin. p70S6K phosphotransferase activity changes during the cell cycle, and increases 20-fold in G1 cells released from G0. It has been linked with the development of diet-induced obesity, colon adenocarcinoma and breast cancer. It is often overexpressed in cancer (e.g., breast cancer) and linked to metabolic diseases like obesity and diabetes. This description may include information annotated by UniProt and/or Google AI.