Product Name:
p38d-pY182
Product Number:
ab-pk743
Target Full Name: Mitogen-activated protein-serine kinase p38 delta; Mitogen-activated protein kinase 13
Target Alias: MAP kinase p38 delta; MAPK13; Mitogen-activated protein kinase p38 delta; MK13; P38 MAPK-delta;p38delta; PRKM13; Stress-activated protein kinase-4; SAPK4; p38d MAPK; ENSG00000156711
Product Type Specific: p38-delta (MAPK13) protein kinase phosphosite-specific antibody
Antibody Code: PK743
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y182
Protein UniProt: O15264
Protein SigNET: p38d
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human p38d MAPK (MAPK13) sequence peptide Cat. No.: PE-04AEJ99
Antibody Immunogen Sequence: MTG(pY)VVT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M179 to T185. Y182 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major in vivo phosphorylation sites in p38d (≥986 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). p38d MAPK is known to be phosphorylated at this site in vitro by MKK3 (MAP2K3, MEK3), MKK4 (MAP2K4), and MKK6 (MAP2K6, MEK6).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Strong immunoreactivity with recombinant human p38d on protein dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Frog | Zebra fish | Fruit fly | Nematode worm.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-45 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with with recombinant humanp38b, but not p38a or p38g. No significant cross-reactive proteins detected in Jurkat cells.
Related Product 1: p38d MAPK (179-185) pY182 antibody blocking peptide (Cat. No.: PE-04BAR00)
Related Product 2: p38SelectideA kinase substrate peptide (Cat. No.: PE-14CAC90)
Related Product 3: p38Subtide kinase substrate peptide (Cat. No.: PE-01BIK99)
Related Product 4: p38d expression antibody (Cat. No.: AB-NK121-2)
Related Product 5: p38d expression antibody (Cat. No.: AB-NK121-3)
Related Product 6: p38d expression antibody (Cat. No.: AB-NK121-4)
Related Product 7: p38d T180+Y182 phosphorylation antibody (Cat. No.: AB-PK742)
Related Product 8: p38d Y182 phosphorylation antibody (Cat. No.: AB-PK743)
Related Product 9: p38d S261+T265 phosphorylation antibody (Cat. No.: AB-PK888)
Related Product 10: p38d T180+Y182 phosphorylation antibody (Cat. No.: AB-PK960)
Scientific Background: p38d (MAPK13) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is essential for MAP kinase signalling that can be induced via physical stress or proinflammatory cytokines. p38d is activated by both proinflammatory cytokines (e.g., TNF-alpha, IL-1-beta), and chemical stress and environmental stress (UV radiation). p38b is directly activated by phosphorylation at Tyr-182 and probably Thr-180 by MKK3 (MAP2K3, MEK3) and MKK6 (MAP2K6, MEK6). p38d signalling can lead to the activation of the transcription factors ELK1 and ATF2, and other kinases such as MAPKAPK2. It can affect proliferation, differentiation, transcription regulation, translation regulation, cytoskeleton regulation, and development. Insulin secretion and pancreatic beta cell survival can be regulated by this kinase. p38d can be a positive or negative regulator of the above processes depending on the cell context. It has also been implicated in promoting heart hypertrophy. Increased expression of p38d has been associated with increases proliferation and metastatic progression. It is also hypermethylated in some cancer cell lines. The rare liver cancer Cholangiocarcinoma (CCA, CC) has been noted to have a significant up-regulation of p38d compared to normal bile duct tissue. It can promote cancer progression, particularly in breast cancer, where it contributes to tumour cell proliferation, and lung metastasis. This description may include information annotated by UniProt and/or Google AI.