Product Name:
p38d-pY182
Product Number:
ab-pk743
Target Full Name: Mitogen-activated protein-serine kinase p38 delta; Mitogen-activated protein kinase 13
Target Alias: MAP kinase p38 delta; MAPK13; Mitogen-activated protein kinase p38 delta; MK13; P38 MAPK-delta;p38delta; PRKM13; Stress-activated protein kinase-4; SAPK4; p38d MAPK; ENSG00000156711
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK743
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y182
Protein UniProt: O15264
Protein SigNET: O15264
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human p38d MAPK (MAPK13) sequence peptide Cat. No.: PE-04AEJ99
Antibody Immunogen Sequence: MTG(pY)VVT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M179 to T185; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues M179 to T185; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Strong immunoreactivity with recombinant human p38d on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-45 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with with recombinant humanp38b, but not p38a or p38g. No significant cross-reactive proteins detected in Jurkat cells.
Related Product 1: p38d-pY182 blocking peptide
Related Product 2: p38 pan-specific antibody (Cat. No.: AB-NK120-5)
Related Product 3: p38d-1 pan-specific antibody (Cat. No.: AB-NK121-2)
Related Product 4: p38d-2 pan-specific antibody (Cat. No.: AB-NK121-3)
Related Product 5: p38d-3 pan-specific antibody (Cat. No.: AB-NK121-4)
Related Product 6: p38d-pT180+pY182 phosphosite-specific antibody (Cat. No.: AB-PK742)
Related Product 7: p38SelectideA - p38a MAPK (MAPK14) protein kinase substrate peptide
Related Product 8: p38Subtide - p38a MAPK (MAPK14) protein kinase substrate peptide
Related Product 9: p38 MAPK KinSub - p38 MAPK peptide substrate
Scientific Background: p38d (MAPK13) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is essential for MAP kinase signalling that can be induced via physical stress or proinflammatory cytokines. It is activated by phosphorylation at Y182 and probably T180 by (MKK3 (MAP2K3) and MKK6 (MAP2K6). p38d signalling can be induced via physical stress or proinflammatory cytokines resulting in the activation of the transcription factors ELK1 and ATF2. It can affect proliferation, differentiation, transcription regulation, translation regulation, cytoskeleton regulation, insulin secretion, cell survival and development. p38d can be a positive or negative regulator of the above processes depending on the cell context. Increased expression of p38d has been associated with increases proliferation and metastatic progression. It is also hypermethylated in some cancer cell lines. The rare liver cancer Cholangiocarcinoma (CCA, CC) has been noted to have a significant up-regulation of p38d compared to normal bile duct tissue. Insulin secretion and pancreatic beta cell survival can be regulated by p38d.
