Product Name:
p38a-pT180+Y182
Product Number:
ab-pk740
Target Full Name: Mitogen-activated protein-serine kinase p38 beta; Mitogen-activated protein kinase 11
Target Alias: MAPK11; MK11; P38 MAPK-beta; P38-2; P38b; PRKM11; SAPK2; Stress-activated protein kinase-2; p28b MARPK; p38Beta; CCDS14090.1; Q15759; ENSG00000185386
Product Type Specific: p38-beta MAPK (MAPK11) protein kinase phosphosite-specific antibody
Antibody Code: PK740
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T180+Y182
Protein UniProt: Q15759
Protein SigNET: p38a
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human p38a/b MAPK (MAPK14) sequence peptide Cat. No.: PE-04ADE99
Antibody Immunogen Sequence: M(pT)G(pY)VAT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M179 to T185; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T180 and Y182 phosphorylation is stimulatory for phosphotransferase activity. These are two of the major in vivo phosphorylation sites in p38a (≥1578 and ≥3744 reports, respectively, by mass spectrometry). p38a MAPK is known to be phosphorylated in vitro at T180 by ASK1 (MAP3K5), MEKK6 (MAP3K6, ASK2), MKK3 (MAP2K3, MEK3), MKK4 (MAP2K4, MEK4), MKK6 (MAP2K6, MEK6), p38a MAPK (MAPK14), PBK (TOPK), and at Y182 by ASK1 (MAP3K5), MEKK6 (MAP3K6, ASK2), MKK3 (MAP2K3, MEK3), MKK4 (MAP2K4, MEK4), MKK6 (MAP2K6, MEK6), p38a MAPK (MAPK14), PBK (TOPK), and Ret.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Nematode worm.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 38-43 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in Jurkat and HepG2 cells. A weak 80 kDa protein was detected in MCF7 cells.
Related Product 1: p38a (179-185) pT180+pY182 antibody blocking peptide (Cat. No.: PE-04ADE99)
Related Product 2: p38SelectideA kinase substrate peptide (Cat. No.: PE-14CAC90)
Related Product 3: p38Subtide kinase substrate peptide (Cat. No.: PE-01BIK99)
Related Product 4: p38b expression antibody (Cat. No.: AB-NK248-1)
Related Product 5: p38b expression antibody (Cat. No.: AB-NK248-2)
Related Product 6: p38a T180+Y182 phosphorylation antibody (Cat. No.: AB-PK740)
Related Product 7: p38b T180+Y182 phosphorylation antibody (Cat. No.: AB-PK741)
Related Product 8: p38 Y182 phosphorylation antibody (Cat. No.: AB-PK959)
Scientific Background: p38b (MAPK11) is a protein-serine/threonine kinase that is a member of the CMGC group of protein kinases in the MAPK family, and p38 subfamily. This kinase is moderate to highly expressed in most tested human tissues. p38b is activated by both proinflammatory cytokines (e.g., TNF-alpha, IL-1-beta), and chemical stress and environmental stress (UV radiation). p38b is directly activated by phosphorylation at Tyr-182 and probably Thr-180 by MKK3 (MAP2K3, MEK3) and MKK6 (MAP2K6, MEK6). Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase. Some of the targets of p38b, like p38a, are downstream kinases such as MSK1 (RPS6KA5) and MSK2 (RPS6KA4), which play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery.Two other kinase targets, MAPKAPK2 (MK2) and MAPKAPK3 (MK3), participate in the control of gene expression mostly at the post-transcriptional level. MKNK1/MNK1 and MKNK2/MNK2 are two other p38-activated protein kinases that regulate protein synthesis. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPK, including ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53, MEF2C and MEF2A. While part of the broader p38 family, p38dis distinguished by its specific tissue distribution and distinct regulatory roles in cell differentiation, apoptosis, and tumour development. Unlike the ubiquitous p38a and p38b isoforms, p38d is primarily expressed in specific tissues, including the glandular tissue (salivary, pituitary, and adrenal glands), lung, kidney, and testis. Alternatively spliced transcript variants encoding the same protein have been observed. p38b has been linked with the development of inflammation and autoimmunity, diabetes and lung neuroendocrine carcinomas. This description may include information annotated by UniProt and/or Google AI.