Product Name:
OSR1-pT185
Product Number:
ab-pk738
Target Full Name: Serine/threonine-protein kinase OSR1
Target Alias: OXSR1
Product Type Specific: OSR1 protein kinase phosphosite-specific antibody
Antibody Code: PK738
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T185
Protein UniProt: O95747
Protein SigNET: OSR1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human OSR1 (OXSR1) sequence peptide Cat. No.: PE-04AOG99
Antibody Immunogen Sequence: VRK(pT)FVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V182 to G188. T185 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major in vivo phosphorylation sites in OSR1 (≥50 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). OSR1 is known to be phosphorylated at this site in vitro by Wnk1 (PRKWNK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 58-62 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: In HeLa cells, phenylarsine oxide (PAO) decreases 75 kDa protein detection. In Jurkat cells, phenylarsine oxide (PAO) increases 65 kDa protein, and phenylarsine oxide (PAO) decreases 75 kDa protein. In MCF7 cells, insulin increases detection of a very strong 50 kDa cross-reactive protein.
Related Product 1: OSR1 (182-188) pT185 antibody blocking peptide (Cat. No.: PE-04AOG99)
Related Product 2: SLC12A2 (198-217) KinSub - SLC12A2-derived peptide substrate; CATCHtide protein kinase substrate peptide
Scientific Background: OSR1 (OXSR1) is a protein-serine/threonine kinase of the STE group and STE20 family. It is activated by stress, and may mediate actin cytoskeleton rearrangement. Phosphorylation of OSR1 at T185 (in the kinase activation loop) and S325 are stimulatory for phosphotransferase activity. A K46A mutation in OSR1 led to the inhibition of autophosphorylation and it prevented activation of phosphotransferase activity. The K36M mutation was noted to prevent phosphorylation of RELT, RELL1, and RELL2, though it is possibility that other kinases in the solution may have been able to still generate a complex with OSR1. In gastric cancer, OSR1 is believed to be methylated in the promoter region inducing down-regulation of gene transcription, leading to disease. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. This description may include information annotated by UniProt and/or Google AI.