Product Name:
OSR1-pT185
Product Number:
ab-pk738
Target Full Name: Serine/threonine-protein kinase OSR1
Target Alias: OXSR1
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK738
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T185
Protein UniProt: O95747
Protein SigNET: O95747
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human OSR1 (OXSR1) sequence peptide Cat. No.: PE-04AOG99
Antibody Immunogen Sequence: VRK(pT)FVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V182 to G188; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues V182 to G188; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 58-62 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: In HeLa cells, phenylarsine oxide (PAO) decreases 75 kDa protein detection. In Jurkat cells, phenylarsine oxide (PAO) increases 65 kDa protein, and phenylarsine oxide (PAO) decreases 75 kDa protein. In MCF7 cells, insulin increases detection of a very strong 50 kDa cross-reactive protein.
Related Product 1: OSR1-pT185 blocking peptide
Related Product 2: SLC12A2 (198-217) KinSub - SLC12A2-derived peptide substrate; CATCHtide protein kinase substrate peptide
Scientific Background: OSR1 (OXSR1) is a protein-serine/threonine kinase of the STE group and STE20 family. It is activated by stress, and may mediate actin cytoskeleton rearrangement. Phosphorylation of OSR1 at T185 (in the kinase activation loop) and S325 are stimulatory for phosphotransferase activity. A K46A mutation in OSR1 led to the inhibition of autophosphorylation and it prevented activation of phosphotransferase activity. The K36M mutation was noted to prevent phosphorylation of RELT, RELL1, and RELL2, though it is possibility that other kinases in the solution may have been able to still generate a complex with OSR1. In gastric cancer, OSR1 is believed to be methylated in the promoter region inducing down-regulation of gene transcription, leading to disease. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
