Product Name:
NuaK1-pT211
Product Number:
ab-pk737
Target Full Name: NUAK family SNF1-like kinase 1; AMPK-related protein kinase 5
Target Alias: AMP-activated protein kinase family member 5; ARK5; KIAA053; KIAA0537; Kinase NuaK1; NUAK family, SNF1-like kinase, 1; NUAK2; Probable KIAA0537; ENSG00000074590
Product Type Specific: NuaK1 protein kinase phosphosite-specific antibody
Antibody Code: PK737
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T211
Protein UniProt: O60285
Protein SigNET: NuaK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human NuaK1 (ARK5)/Nuak2 sequence peptide Cat. No.: PE-04AKS99
Antibody Immunogen Sequence: FLQ(pT)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F208 to G214; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T211 phosphorylation stimulates phosphotransferase activity. This is a minor in vivo phosphorylation sites in Nuak1 (≥1 report from high throughput mass spectrometry studies recorded in PhosphoSitePlus). NuaK1 is known to be phosphorylated at this site in vitro by LKB1 (STK11), and NDR2 (STK38L).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-76 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2 cells.
Related Product 1: NUAK1 (208-214) pT211 antibody blocking peptide (Cat. No.: PE-04AKS99)
Related Product 2: Cdc25C (205-225), KinSub - Cdc25C phosphatase (V205-R225, human) peptide; Chktide peptide
Scientific Background: NuaK1 ((NUAK family SNF1-like kinase 1;ARK5) is a 74-76 kDa protein-serine/threonine kinase of the CAMK group and CAMKL family. It is a key member of the AMPK family involved in energy stress responses, and mediates cell proliferation, senescence, cell ploidy levels, and cell adhesion. It is Involved in tolerance to glucose starvation. It is activated by phosphorylation at Thr-211 by LKB1 and Ser-600 by Akt. A T211A NuaK1 mutation can inhibit LKB1/LKB1-mediated activation and phosphorylation of NuaK1, while ablating induction of senescence. The I400K and L401K mutations can lead to interrupted NuaK1 complex with PPP1CB, and inhibiting regulation of PP1 activity. It suppresses Fas-induced apoptosis by phosphorylation of CASP6, and suppresses the activation of the caspase and the subsequent cleavage of CFLAR. It phosphorylates ATM. Ploidy by NuaK1 is mediated through the phosphorylation of Ser-464 of LATS1. Cell adhesion control by NuaK1 is mediated via the Myosin Phosphatase 1 (PP1) complex and contributes to DNA repair by regulating p53 and CDKN1A (p21). Phosphorylation via PKB/Akt1 can be prevented through the S600A mutation. It functions as a tumor malignancy-associated factor, promoting survival under nutrient starvation and enabling cancer cell invasion and metastasis. Elevated levels of NUAK1 are associated with poor prognosis in various malignancies, including glioma and ovarian cancer. This description may include information annotated by UniProt and/or Google AI.