Product Name:
NuaK1-pT211
Product Number:
ab-pk737
Target Full Name: NUAK family SNF1-like kinase 1; AMPK-related protein kinase 5
Target Alias: AMP-activated protein kinase family member 5; ARK5; KIAA053; KIAA0537; Kinase NuaK1; NUAK family, SNF1-like kinase, 1; NUAK2; Probable KIAA0537; ENSG00000074590
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK737
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T211
Protein UniProt: O60285
Protein SigNET: O60285
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human NuaK1 (ARK5)/Nuak2 sequence peptide Cat. No.: PE-04AKS99
Antibody Immunogen Sequence: FLQ(pT)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F208 to G214; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues F208 to G214; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-76 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2 cells.
Related Product 1: NuaK1-pT211 blocking peptide
Related Product 2: Cdc25C (205-225), KinSub - Cdc25C phosphatase (V205-R225, human) peptide; Chktide peptide
Scientific Background: NuaK1 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It mediates cell proliferation, tumour progression, senescence, cell ploidy levels, and cell adhesion. It is Involved in tolerance to glucose starvation. It is activated by phosphorylation at T211 and S600. A T211A NuaK1 mutation can inhibit LKB1/LKB1-mediated activation and phosphorylation of NuaK1, while ablating induction of senescence. The I400K and L401K mutations can lead to interrupted NuaK1 complex with PPP1CB, and inhibiting regulation of PP1 activity. It suppresses Fas-induced apoptosis by phosphorylation of CASP6, and suppresses the activation of the caspase and the subsequent cleavage of CFLAR. It phosphorylates ATM. Ploidy by NuaK1 is mediated through the phosphorylation of S464 of LATS1. Cell adhesion control by NuaK1 is mediated via PP1 (protein phosphatase 1). Phosphorylation via PKB/Akt1 can be prevented through the S600A mutation.
