Product Name:
NLK-pT298
Product Number:
ab-pk736
Target Full Name: Serine/threonine protein kinase NLK
Target Alias: Kinase NLK; LAK1; Nemo-like kinase
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK736
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T298
Protein UniProt: Q9UBE8
Protein SigNET: Q9UBE8
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human NLK sequence peptide Cat. No.: PE-04AOF99
Antibody Immunogen Sequence: RHM(pT)QEV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R295 to V301; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues R295 to V301; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.25 / 1.0 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 64-68 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer, although a strong background >75 kDa was observed.
Related Product 1: NLK-pT298 blocking peptide
Scientific Background: NLK is a protein-serine/threonine kinase that is a member of the CMGC group of protein kinases in the MAPK family, and nmo subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. NLK is activated by phosphorylation at tyrosine and threonine, and by by activin. NLK plays a role in cell fate determination and is required for the differentiation of bone marrow stromal cells. NLK also suppresses a wide range of transcription factors such as CREB by phosphorylation of the co-activator CREBBP. NLK expression increases the number of apoptotic cells but does not induce obvious cell cycle arrest. NLK suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. NLK has been linked with the development of glioblastoma multiforme (GM). Overexpression of NLK is associated with suppressed human colon cancer cell line growth. NLK inhibits human adenosquamous carcinoma cells proliferation and block cell cycle progression to S phase.