Product Name:
Nek7-pT191+S195
Product Number:
ab-pk735
Target Full Name: NIMA (never-in-mitosis)-related protein-serine kinase 7
Target Alias: Kinase Nek7; NIMA (never in mitosis gene a)-related kinase 7; NIMA-related serine/threonine kinase Nek7
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK735
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T191+S195
Protein UniProt: Q8TDX7
Protein SigNET: Q8TDX7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Nek7 sequence peptide Cat. No.: PE-04AHR99
Antibody Immunogen Sequence: (pT)AAH(pS)LVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T191 to G198; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues T191 to G198; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 34-37 kDa.
Related Product 1: Nek7-pT191+S195 blocking peptide
Scientific Background: Nek7 is a protein-serine/threonine kinase that is a member of the Other group of protein kinases in the Nek family. This kinase is highly expressed and widely distributed in most tested human tissues. Nek7 is a member of the NIMA family of serine/threonine kinases. In contrast to the other documented NimA-related kinases, Nek7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies indicate that Nek7 is cytoplasmic and located on chromosome 1. The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) T412, was purified from rat liver and identified as Nek7. Nek7 phosphotransferase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner. Nek7 has been linked with the development of ovarian serous carcinomas. Nek7 has been significantly associated with certain clinicopathologic indices in gallbladder carcinomas. The high level expression of Nek7, FoxM1 and Plk1 was significantly associated with shorter overall survival time in 76 specimens of gallbladder carcinomas, pericarcinomas and normal tissues (PubMEd: 23359173).
